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Tracking the cell hierarchy in the human intestine using biochemical signatures derived by mid-infrared microspectroscopy.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Tracking the cell hierarchy in the human intestine using biochemical signatures derived by mid-infrared microspectroscopy. / Walsh, Michael John; Hammiche, Azzedine; Fellous, Tariq G. et al.
In: Stem Cell Research, Vol. 3, No. 1, 07.2009, p. 15-27.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Walsh, MJ, Hammiche, A, Fellous, TG, Nicholson, JM, Cotte, M, Susini, J, Fullwood, NJ, Martin-Hirsch, PL, Alison, MR & Martin, FL 2009, 'Tracking the cell hierarchy in the human intestine using biochemical signatures derived by mid-infrared microspectroscopy.', Stem Cell Research, vol. 3, no. 1, pp. 15-27. https://doi.org/10.1016/j.scr.2009.02.003

APA

Walsh, M. J., Hammiche, A., Fellous, T. G., Nicholson, J. M., Cotte, M., Susini, J., Fullwood, N. J., Martin-Hirsch, P. L., Alison, M. R., & Martin, F. L. (2009). Tracking the cell hierarchy in the human intestine using biochemical signatures derived by mid-infrared microspectroscopy. Stem Cell Research, 3(1), 15-27. https://doi.org/10.1016/j.scr.2009.02.003

Vancouver

Walsh MJ, Hammiche A, Fellous TG, Nicholson JM, Cotte M, Susini J et al. Tracking the cell hierarchy in the human intestine using biochemical signatures derived by mid-infrared microspectroscopy. Stem Cell Research. 2009 Jul;3(1):15-27. doi: 10.1016/j.scr.2009.02.003

Author

Walsh, Michael John ; Hammiche, Azzedine ; Fellous, Tariq G. et al. / Tracking the cell hierarchy in the human intestine using biochemical signatures derived by mid-infrared microspectroscopy. In: Stem Cell Research. 2009 ; Vol. 3, No. 1. pp. 15-27.

Bibtex

@article{84d94818e306430f85915a1496050dd7,
title = "Tracking the cell hierarchy in the human intestine using biochemical signatures derived by mid-infrared microspectroscopy.",
abstract = "Markers of gastrointestinal (GI) stem cells remain elusive. We employed synchrotron Fourier-transform infrared (FTIR) microspectroscopy to derive mid-infrared (IR) spectra along the length of human GI crypts. Tissue sections (10-μm thick) were floated onto BaF2 windows and image maps were acquired of small intestine and large bowel crypts in transmission mode with an aperture of ≤ 10 μm × 10 μm. Counting upwards in a step-size (≤ 10 μm) fashion from the crypt base, IR spectra were extracted from the image maps and each spectrum corresponding to a particular location was identified. Spectra were analyzed using principal component analysis plus linear discriminant analysis. Compared to putative crypt base columnar/Paneth cells, those assigned as label-retaining cells were chemically more similar to putative large bowel stem cells and, the small intestine transit-amplifying cells were closest to large bowel transit-amplifying cells; interestingly, the base of small intestine crypts was the most chemically-distinct. This study suggests that in the complex cell lineage of human GI crypts, chemical similarities as revealed by FTIR microspectroscopy between regions putatively assigned as stem cell, transit-amplifying and terminally-differentiated facilitates identification of cell function.",
author = "Walsh, {Michael John} and Azzedine Hammiche and Fellous, {Tariq G.} and Nicholson, {James M.} and Marine Cotte and Jean Susini and Fullwood, {Nigel J.} and Martin-Hirsch, {Pierre L.} and Alison, {Malcolm R.} and Martin, {Frank L.}",
year = "2009",
month = jul,
doi = "10.1016/j.scr.2009.02.003",
language = "English",
volume = "3",
pages = "15--27",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Tracking the cell hierarchy in the human intestine using biochemical signatures derived by mid-infrared microspectroscopy.

AU - Walsh, Michael John

AU - Hammiche, Azzedine

AU - Fellous, Tariq G.

AU - Nicholson, James M.

AU - Cotte, Marine

AU - Susini, Jean

AU - Fullwood, Nigel J.

AU - Martin-Hirsch, Pierre L.

AU - Alison, Malcolm R.

AU - Martin, Frank L.

PY - 2009/7

Y1 - 2009/7

N2 - Markers of gastrointestinal (GI) stem cells remain elusive. We employed synchrotron Fourier-transform infrared (FTIR) microspectroscopy to derive mid-infrared (IR) spectra along the length of human GI crypts. Tissue sections (10-μm thick) were floated onto BaF2 windows and image maps were acquired of small intestine and large bowel crypts in transmission mode with an aperture of ≤ 10 μm × 10 μm. Counting upwards in a step-size (≤ 10 μm) fashion from the crypt base, IR spectra were extracted from the image maps and each spectrum corresponding to a particular location was identified. Spectra were analyzed using principal component analysis plus linear discriminant analysis. Compared to putative crypt base columnar/Paneth cells, those assigned as label-retaining cells were chemically more similar to putative large bowel stem cells and, the small intestine transit-amplifying cells were closest to large bowel transit-amplifying cells; interestingly, the base of small intestine crypts was the most chemically-distinct. This study suggests that in the complex cell lineage of human GI crypts, chemical similarities as revealed by FTIR microspectroscopy between regions putatively assigned as stem cell, transit-amplifying and terminally-differentiated facilitates identification of cell function.

AB - Markers of gastrointestinal (GI) stem cells remain elusive. We employed synchrotron Fourier-transform infrared (FTIR) microspectroscopy to derive mid-infrared (IR) spectra along the length of human GI crypts. Tissue sections (10-μm thick) were floated onto BaF2 windows and image maps were acquired of small intestine and large bowel crypts in transmission mode with an aperture of ≤ 10 μm × 10 μm. Counting upwards in a step-size (≤ 10 μm) fashion from the crypt base, IR spectra were extracted from the image maps and each spectrum corresponding to a particular location was identified. Spectra were analyzed using principal component analysis plus linear discriminant analysis. Compared to putative crypt base columnar/Paneth cells, those assigned as label-retaining cells were chemically more similar to putative large bowel stem cells and, the small intestine transit-amplifying cells were closest to large bowel transit-amplifying cells; interestingly, the base of small intestine crypts was the most chemically-distinct. This study suggests that in the complex cell lineage of human GI crypts, chemical similarities as revealed by FTIR microspectroscopy between regions putatively assigned as stem cell, transit-amplifying and terminally-differentiated facilitates identification of cell function.

U2 - 10.1016/j.scr.2009.02.003

DO - 10.1016/j.scr.2009.02.003

M3 - Journal article

VL - 3

SP - 15

EP - 27

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

IS - 1

ER -