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ULTRASTRUCTURAL DISTRIBUTION OF ENDOGENOUS IMMUNOGLOBULIN-G IN HUMAN TERM AMNIOCHORION

Research output: Contribution to journalJournal articlepeer-review

Published
<mark>Journal publication date</mark>08/1995
<mark>Journal</mark>Cell and Tissue Research
Issue number2
Volume281
Number of pages8
Pages (from-to)367-374
Publication StatusPublished
<mark>Original language</mark>English

Abstract

Maternal immunoglobulin-G (IgG) is known to be transported across the placental syncytiotrophoblast during the period when the human fetus is incapable of manufacturing these defensive molecules. In this study we investigated the possible role of the amniochorion, that surrounds the amniotic cavity in which the fetus lies, in the transfer of immunoglobulin. Endogenous IgG was localised in the amniochorion by confocal immunofluorescence microscopy and by ultrastructural labelling of ultrathin frozen tissue sections using the protein A-gold technique. Immunoreactivity was identified in the extracellular matrix tissues and necrotic amniotic epithelial cells. Healthy amniotic epithelial cells and cytotrophoblast cells of the chorion laeve were devoid of endogenous IgG. These results suggest a possible nonspecific paracellular transport pathway between cytotrophoblast cells, which may conceivably contribute to the acquisition of passive immunity by the fetus, and offer a rational explanation for the presence of small quantities of maternal IgG in the amniotic fluid.