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Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium. / Gray, N. D.; Howarth, R.; Pickup, R. W. et al.
In: Applied and Environmental Microbiology, Vol. 66, No. 10, 01.10.2000, p. 4518-4522.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Gray, ND, Howarth, R, Pickup, RW, Jones, JG & Head, IM 2000, 'Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium', Applied and Environmental Microbiology, vol. 66, no. 10, pp. 4518-4522. https://doi.org/10.1128/AEM.66.10.4518-4522.2000

APA

Gray, N. D., Howarth, R., Pickup, R. W., Jones, J. G., & Head, I. M. (2000). Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium. Applied and Environmental Microbiology, 66(10), 4518-4522. https://doi.org/10.1128/AEM.66.10.4518-4522.2000

Vancouver

Gray ND, Howarth R, Pickup RW, Jones JG, Head IM. Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium. Applied and Environmental Microbiology. 2000 Oct 1;66(10):4518-4522. doi: 10.1128/AEM.66.10.4518-4522.2000

Author

Gray, N. D. ; Howarth, R. ; Pickup, R. W. et al. / Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium. In: Applied and Environmental Microbiology. 2000 ; Vol. 66, No. 10. pp. 4518-4522.

Bibtex

@article{a9d5e4c1e0a94995a3332b7070782b01,
title = "Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium",
abstract = "Combined microautoradiography and fluorescence in situ hybridization (FISH) was used to investigate carbon metabolism in uncultured bacteria from the genus Achromatium. All of the Achromatium species identified in a freshwater sediment from Rydal Water, Cumbria, United Kingdom, which were distinguishable only by FISH, assimilated both [14C]bicarbonate and [14C]acetate. This extends previous findings that Achromatium spp. present at another location could only utilize organic carbon sources. Achromatium spp., therefore, probably exhibit a range of physiologies, i.e., facultative chemolithoautotrophy, mixotrophy, and chemoorganoheterotrophy, similar to other large sulfur bacteria (e.g., Beggiatoa spp.).",
author = "Gray, {N. D.} and R. Howarth and Pickup, {R. W.} and Jones, {J. G.} and Head, {I. M.}",
year = "2000",
month = oct,
day = "1",
doi = "10.1128/AEM.66.10.4518-4522.2000",
language = "English",
volume = "66",
pages = "4518--4522",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "10",

}

RIS

TY - JOUR

T1 - Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium

AU - Gray, N. D.

AU - Howarth, R.

AU - Pickup, R. W.

AU - Jones, J. G.

AU - Head, I. M.

PY - 2000/10/1

Y1 - 2000/10/1

N2 - Combined microautoradiography and fluorescence in situ hybridization (FISH) was used to investigate carbon metabolism in uncultured bacteria from the genus Achromatium. All of the Achromatium species identified in a freshwater sediment from Rydal Water, Cumbria, United Kingdom, which were distinguishable only by FISH, assimilated both [14C]bicarbonate and [14C]acetate. This extends previous findings that Achromatium spp. present at another location could only utilize organic carbon sources. Achromatium spp., therefore, probably exhibit a range of physiologies, i.e., facultative chemolithoautotrophy, mixotrophy, and chemoorganoheterotrophy, similar to other large sulfur bacteria (e.g., Beggiatoa spp.).

AB - Combined microautoradiography and fluorescence in situ hybridization (FISH) was used to investigate carbon metabolism in uncultured bacteria from the genus Achromatium. All of the Achromatium species identified in a freshwater sediment from Rydal Water, Cumbria, United Kingdom, which were distinguishable only by FISH, assimilated both [14C]bicarbonate and [14C]acetate. This extends previous findings that Achromatium spp. present at another location could only utilize organic carbon sources. Achromatium spp., therefore, probably exhibit a range of physiologies, i.e., facultative chemolithoautotrophy, mixotrophy, and chemoorganoheterotrophy, similar to other large sulfur bacteria (e.g., Beggiatoa spp.).

U2 - 10.1128/AEM.66.10.4518-4522.2000

DO - 10.1128/AEM.66.10.4518-4522.2000

M3 - Journal article

C2 - 11010908

AN - SCOPUS:0033775691

VL - 66

SP - 4518

EP - 4522

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 10

ER -