TY - JOUR

T1 - Using X-ray computed tomography to explore the role of abscisic acid in moderating the impact of soil compaction on root system architecture

AU - Tracy, Saoirse R.

AU - Black, Colin R.

AU - Roberts, Jeremy A.

AU - Dodd, Ian C.

AU - Mooney, Sacha J.

PY - 2015/2

Y1 - 2015/2

N2 - Background and Airns: Understanding how soil compaction affects root system architecture (RSA) and root deployment within soil is critical to maximise crop growth. This study examined the role of abscisic acid (ABA) in mediating root responses to soil compaction using tomato genotypes with differing endogenous ABA concentrations.Methods: Plants of the wild-type tomato genotype (Solanum lycopersicum L. cv. Ailsa Craig) and its ABA-deficient mutant notabilis, of uniform developmental stage, were transplanted to columns containing a loamy sand soil at bulk densities of 1.2, 1.4 and 1.6 Mg m(-3). Fourteen days after transplanting (DAT), an X-ray mu CT scanner acquired non-destructive 3-D images of RSA. Destructive analysis of RSA was undertaken using WinRHIZO((R)) 2-D scanning equipment.Key results: Increased bulk density decreased root volume, surface area, rooting depth and lateral root number (P<0.05), which adversely affected RSA and the subsequent volume of soil explored. The ABA-deficient mutant notabilis displayed a poor rooting phenotype with reduced root volume, surface area and lateral roots at all bulk densities.Conclusions: The response of RSA to soil compaction in situ differed between the ABA-deficient mutant genotypes of tomato. The differences in rooting phenotype between genotypes suggest that endogenous ABA concentration has a positive influence on RSA when roots encounter compacted soil. (C) 2014 Elsevier B.V. All rights reserved.

AB - Background and Airns: Understanding how soil compaction affects root system architecture (RSA) and root deployment within soil is critical to maximise crop growth. This study examined the role of abscisic acid (ABA) in mediating root responses to soil compaction using tomato genotypes with differing endogenous ABA concentrations.Methods: Plants of the wild-type tomato genotype (Solanum lycopersicum L. cv. Ailsa Craig) and its ABA-deficient mutant notabilis, of uniform developmental stage, were transplanted to columns containing a loamy sand soil at bulk densities of 1.2, 1.4 and 1.6 Mg m(-3). Fourteen days after transplanting (DAT), an X-ray mu CT scanner acquired non-destructive 3-D images of RSA. Destructive analysis of RSA was undertaken using WinRHIZO((R)) 2-D scanning equipment.Key results: Increased bulk density decreased root volume, surface area, rooting depth and lateral root number (P<0.05), which adversely affected RSA and the subsequent volume of soil explored. The ABA-deficient mutant notabilis displayed a poor rooting phenotype with reduced root volume, surface area and lateral roots at all bulk densities.Conclusions: The response of RSA to soil compaction in situ differed between the ABA-deficient mutant genotypes of tomato. The differences in rooting phenotype between genotypes suggest that endogenous ABA concentration has a positive influence on RSA when roots encounter compacted soil. (C) 2014 Elsevier B.V. All rights reserved.

KW - Abscisic acid (ABA)

KW - Root system architecture

KW - Soil compaction

KW - Solanum lycopersicum

KW - Computed TomographyROIRegion of interest

KW - TOMATO SOLANUM-LYCOPERSICON

KW - LOW WATER POTENTIALS

KW - VULGARE L GROWTH

KW - LEAF EXPANSION

KW - SHOOT GROWTH

KW - ABA

KW - PLANT

KW - ETHYLENE

KW - ELONGATION

KW - STRESS

U2 - 10.1016/j.envexpbot.2014.09.003

DO - 10.1016/j.envexpbot.2014.09.003

M3 - Journal article

VL - 110

SP - 11

EP - 18

JO - Environmental and Experimental Botany

JF - Environmental and Experimental Botany

SN - 0098-8472

ER -