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Visceral leishmaniasis in Teresina, n. e. Brazil: towards a DNA probe kit and its adaptation to processing blood-contaminated samples.

Research output: Contribution to journalJournal articlepeer-review

Published
  • R. McNerney
  • I. A. Frame
  • J. A. Vexenat
  • J. A. Fonseca de Castro
  • M. K. Howard
  • R. Dillon
  • M. A. Miles
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<mark>Journal publication date</mark>1/01/1993
<mark>Journal</mark>Archives de l'Institut Pasteur de Tunis
Issue number3-4
Volume70
Number of pages14
Pages (from-to)405-418
Publication StatusPublished
<mark>Original language</mark>English

Abstract

The Lmet2 chemiluminescent DNA probe is a valuable tool for identifying parasites of the Leishmania donovani -complex in sand flies, dogs and human samples. Recent blood meals in sand flies or blood contamination of tissue samples inhibited probe sensitivity, whether radiolabelled or chemiluminescent detection systems were used. Treatment of membranes with protease before hybridisation restored positive signal. Alternatively samples could be lysed with protease and applied to membranes with a vacuum blotting apparatus. The Lmet2 protocol provides the basis for a DNA probe kit that is adaptable for use with a wide range of other probes.