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Visceral leishmaniasis in Teresina, n. e. Brazil: towards a DNA probe kit and its adaptation to processing blood-contaminated samples.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Visceral leishmaniasis in Teresina, n. e. Brazil : towards a DNA probe kit and its adaptation to processing blood-contaminated samples. / McNerney, R.; Frame, I. A.; Vexenat, J. A. et al.

In: Archives de l'Institut Pasteur de Tunis, Vol. 70, No. 3-4, 01.01.1993, p. 405-418.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

McNerney, R, Frame, IA, Vexenat, JA, Fonseca de Castro, JA, Howard, MK, Dillon, R & Miles, MA 1993, 'Visceral leishmaniasis in Teresina, n. e. Brazil: towards a DNA probe kit and its adaptation to processing blood-contaminated samples.', Archives de l'Institut Pasteur de Tunis, vol. 70, no. 3-4, pp. 405-418. <https://europepmc.org/article/med/7802496>

APA

McNerney, R., Frame, I. A., Vexenat, J. A., Fonseca de Castro, J. A., Howard, M. K., Dillon, R., & Miles, M. A. (1993). Visceral leishmaniasis in Teresina, n. e. Brazil: towards a DNA probe kit and its adaptation to processing blood-contaminated samples. Archives de l'Institut Pasteur de Tunis, 70(3-4), 405-418. https://europepmc.org/article/med/7802496

Vancouver

McNerney R, Frame IA, Vexenat JA, Fonseca de Castro JA, Howard MK, Dillon R et al. Visceral leishmaniasis in Teresina, n. e. Brazil: towards a DNA probe kit and its adaptation to processing blood-contaminated samples. Archives de l'Institut Pasteur de Tunis. 1993 Jan 1;70(3-4):405-418.

Author

McNerney, R. ; Frame, I. A. ; Vexenat, J. A. et al. / Visceral leishmaniasis in Teresina, n. e. Brazil : towards a DNA probe kit and its adaptation to processing blood-contaminated samples. In: Archives de l'Institut Pasteur de Tunis. 1993 ; Vol. 70, No. 3-4. pp. 405-418.

Bibtex

@article{5c55c0f3dc3441a283d398599ee7424f,
title = "Visceral leishmaniasis in Teresina, n. e. Brazil: towards a DNA probe kit and its adaptation to processing blood-contaminated samples.",
abstract = "The Lmet2 chemiluminescent DNA probe is a valuable tool for identifying parasites of the Leishmania donovani -complex in sand flies, dogs and human samples. Recent blood meals in sand flies or blood contamination of tissue samples inhibited probe sensitivity, whether radiolabelled or chemiluminescent detection systems were used. Treatment of membranes with protease before hybridisation restored positive signal. Alternatively samples could be lysed with protease and applied to membranes with a vacuum blotting apparatus. The Lmet2 protocol provides the basis for a DNA probe kit that is adaptable for use with a wide range of other probes.",
author = "R. McNerney and Frame, {I. A.} and Vexenat, {J. A.} and {Fonseca de Castro}, {J. A.} and Howard, {M. K.} and R. Dillon and Miles, {M. A.}",
year = "1993",
month = jan,
day = "1",
language = "English",
volume = "70",
pages = "405--418",
journal = "Archives de l'Institut Pasteur de Tunis",
issn = "0020-2509",
publisher = "Institut Pasteur de Tunis",
number = "3-4",

}

RIS

TY - JOUR

T1 - Visceral leishmaniasis in Teresina, n. e. Brazil

T2 - towards a DNA probe kit and its adaptation to processing blood-contaminated samples.

AU - McNerney, R.

AU - Frame, I. A.

AU - Vexenat, J. A.

AU - Fonseca de Castro, J. A.

AU - Howard, M. K.

AU - Dillon, R.

AU - Miles, M. A.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - The Lmet2 chemiluminescent DNA probe is a valuable tool for identifying parasites of the Leishmania donovani -complex in sand flies, dogs and human samples. Recent blood meals in sand flies or blood contamination of tissue samples inhibited probe sensitivity, whether radiolabelled or chemiluminescent detection systems were used. Treatment of membranes with protease before hybridisation restored positive signal. Alternatively samples could be lysed with protease and applied to membranes with a vacuum blotting apparatus. The Lmet2 protocol provides the basis for a DNA probe kit that is adaptable for use with a wide range of other probes.

AB - The Lmet2 chemiluminescent DNA probe is a valuable tool for identifying parasites of the Leishmania donovani -complex in sand flies, dogs and human samples. Recent blood meals in sand flies or blood contamination of tissue samples inhibited probe sensitivity, whether radiolabelled or chemiluminescent detection systems were used. Treatment of membranes with protease before hybridisation restored positive signal. Alternatively samples could be lysed with protease and applied to membranes with a vacuum blotting apparatus. The Lmet2 protocol provides the basis for a DNA probe kit that is adaptable for use with a wide range of other probes.

M3 - Journal article

C2 - 7802496

AN - SCOPUS:0027639023

VL - 70

SP - 405

EP - 418

JO - Archives de l'Institut Pasteur de Tunis

JF - Archives de l'Institut Pasteur de Tunis

SN - 0020-2509

IS - 3-4

ER -