Final published version
Licence: CC BY: Creative Commons Attribution 4.0 International License
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Visceral leishmaniasis in Teresina, n. e. Brazil : towards a DNA probe kit and its adaptation to processing blood-contaminated samples. / McNerney, R.; Frame, I. A.; Vexenat, J. A. et al.
In: Archives de l'Institut Pasteur de Tunis, Vol. 70, No. 3-4, 01.01.1993, p. 405-418.Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Visceral leishmaniasis in Teresina, n. e. Brazil
T2 - towards a DNA probe kit and its adaptation to processing blood-contaminated samples.
AU - McNerney, R.
AU - Frame, I. A.
AU - Vexenat, J. A.
AU - Fonseca de Castro, J. A.
AU - Howard, M. K.
AU - Dillon, R.
AU - Miles, M. A.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - The Lmet2 chemiluminescent DNA probe is a valuable tool for identifying parasites of the Leishmania donovani -complex in sand flies, dogs and human samples. Recent blood meals in sand flies or blood contamination of tissue samples inhibited probe sensitivity, whether radiolabelled or chemiluminescent detection systems were used. Treatment of membranes with protease before hybridisation restored positive signal. Alternatively samples could be lysed with protease and applied to membranes with a vacuum blotting apparatus. The Lmet2 protocol provides the basis for a DNA probe kit that is adaptable for use with a wide range of other probes.
AB - The Lmet2 chemiluminescent DNA probe is a valuable tool for identifying parasites of the Leishmania donovani -complex in sand flies, dogs and human samples. Recent blood meals in sand flies or blood contamination of tissue samples inhibited probe sensitivity, whether radiolabelled or chemiluminescent detection systems were used. Treatment of membranes with protease before hybridisation restored positive signal. Alternatively samples could be lysed with protease and applied to membranes with a vacuum blotting apparatus. The Lmet2 protocol provides the basis for a DNA probe kit that is adaptable for use with a wide range of other probes.
M3 - Journal article
C2 - 7802496
AN - SCOPUS:0027639023
VL - 70
SP - 405
EP - 418
JO - Archives de l'Institut Pasteur de Tunis
JF - Archives de l'Institut Pasteur de Tunis
SN - 0020-2509
IS - 3-4
ER -