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Whey protein isolate/calcium silicate hydrogels for bone tissue engineering applications – preliminary evaluation in vitro

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Whey protein isolate/calcium silicate hydrogels for bone tissue engineering applications – preliminary evaluation in vitro. / Ivory-Cousins, Tayla; Nurzynska, Aleksandra; Klimek, Katarzyna et al.
In: Materials, Vol. 16, No. 19, 6484, 29.09.2023.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Ivory-Cousins, T, Nurzynska, A, Klimek, K, Baines, D, Truszkiewicz, W, Palka, K & Douglas, T 2023, 'Whey protein isolate/calcium silicate hydrogels for bone tissue engineering applications – preliminary evaluation in vitro', Materials, vol. 16, no. 19, 6484. https://doi.org/10.3390/ma16196484

APA

Ivory-Cousins, T., Nurzynska, A., Klimek, K., Baines, D., Truszkiewicz, W., Palka, K., & Douglas, T. (2023). Whey protein isolate/calcium silicate hydrogels for bone tissue engineering applications – preliminary evaluation in vitro. Materials, 16(19), Article 6484. https://doi.org/10.3390/ma16196484

Vancouver

Ivory-Cousins T, Nurzynska A, Klimek K, Baines D, Truszkiewicz W, Palka K et al. Whey protein isolate/calcium silicate hydrogels for bone tissue engineering applications – preliminary evaluation in vitro. Materials. 2023 Sept 29;16(19):6484. doi: 10.3390/ma16196484

Author

Ivory-Cousins, Tayla ; Nurzynska, Aleksandra ; Klimek, Katarzyna et al. / Whey protein isolate/calcium silicate hydrogels for bone tissue engineering applications – preliminary evaluation in vitro. In: Materials. 2023 ; Vol. 16, No. 19.

Bibtex

@article{8fd28ab7aaec4334968c035dcfacfba6,
title = "Whey protein isolate/calcium silicate hydrogels for bone tissue engineering applications – preliminary evaluation in vitro",
abstract = "Whey protein isolate (WPI) hydrogels are attractive biomaterials for application in bone repair and regeneration. However, their main limitation is low mechanical strength. Therefore, to improve these properties, the incorporation of ceramic phases into hydrogel matrices is currently being performed. In this study, novel whey protein isolate/calcium silicate (WPI/CaSiO3) hydrogel biomaterials were prepared with varying concentrations of a ceramic phase (CaSiO3). The aim of this study was to investigate the effect of the introduction of CaSiO3 to a WPI hydrogel matrix on its physicochemical, mechanical, and biological properties. Our Fourier Transform Infrared Spectroscopy results showed that CaSiO3 was successfully incorporated into the WPI hydrogel matrix to create composite biomaterials. Swelling tests indicated that the addition of 5% (w/v) CaSiO3 caused greater swelling compared to biomaterials without CaSiO3 and ultimate compressive strength and strain at break. Cell culture experiments demonstrated that WPI hydrogel biomaterials enriched with CaSiO3 demonstrated superior cytocompatibility in vitro compared to the control hydrogel biomaterials without CaSiO3. Thus, this study revealed that the addition of CaSiO3 to WPI-based hydrogel biomaterials renders them more promising for bone tissue engineering applications.",
keywords = "whey protein, calcium silicate, bone scaffolds, SEM, FTIR, swelling, osteoblasts, cytocompatibility",
author = "Tayla Ivory-Cousins and Aleksandra Nurzynska and Katarzyna Klimek and Daniel Baines and Wieslaw Truszkiewicz and Krzysztof Palka and Timothy Douglas",
year = "2023",
month = sep,
day = "29",
doi = "10.3390/ma16196484",
language = "English",
volume = "16",
journal = "Materials",
issn = "1996-1944",
publisher = "MDPI AG",
number = "19",

}

RIS

TY - JOUR

T1 - Whey protein isolate/calcium silicate hydrogels for bone tissue engineering applications – preliminary evaluation in vitro

AU - Ivory-Cousins, Tayla

AU - Nurzynska, Aleksandra

AU - Klimek, Katarzyna

AU - Baines, Daniel

AU - Truszkiewicz, Wieslaw

AU - Palka, Krzysztof

AU - Douglas, Timothy

PY - 2023/9/29

Y1 - 2023/9/29

N2 - Whey protein isolate (WPI) hydrogels are attractive biomaterials for application in bone repair and regeneration. However, their main limitation is low mechanical strength. Therefore, to improve these properties, the incorporation of ceramic phases into hydrogel matrices is currently being performed. In this study, novel whey protein isolate/calcium silicate (WPI/CaSiO3) hydrogel biomaterials were prepared with varying concentrations of a ceramic phase (CaSiO3). The aim of this study was to investigate the effect of the introduction of CaSiO3 to a WPI hydrogel matrix on its physicochemical, mechanical, and biological properties. Our Fourier Transform Infrared Spectroscopy results showed that CaSiO3 was successfully incorporated into the WPI hydrogel matrix to create composite biomaterials. Swelling tests indicated that the addition of 5% (w/v) CaSiO3 caused greater swelling compared to biomaterials without CaSiO3 and ultimate compressive strength and strain at break. Cell culture experiments demonstrated that WPI hydrogel biomaterials enriched with CaSiO3 demonstrated superior cytocompatibility in vitro compared to the control hydrogel biomaterials without CaSiO3. Thus, this study revealed that the addition of CaSiO3 to WPI-based hydrogel biomaterials renders them more promising for bone tissue engineering applications.

AB - Whey protein isolate (WPI) hydrogels are attractive biomaterials for application in bone repair and regeneration. However, their main limitation is low mechanical strength. Therefore, to improve these properties, the incorporation of ceramic phases into hydrogel matrices is currently being performed. In this study, novel whey protein isolate/calcium silicate (WPI/CaSiO3) hydrogel biomaterials were prepared with varying concentrations of a ceramic phase (CaSiO3). The aim of this study was to investigate the effect of the introduction of CaSiO3 to a WPI hydrogel matrix on its physicochemical, mechanical, and biological properties. Our Fourier Transform Infrared Spectroscopy results showed that CaSiO3 was successfully incorporated into the WPI hydrogel matrix to create composite biomaterials. Swelling tests indicated that the addition of 5% (w/v) CaSiO3 caused greater swelling compared to biomaterials without CaSiO3 and ultimate compressive strength and strain at break. Cell culture experiments demonstrated that WPI hydrogel biomaterials enriched with CaSiO3 demonstrated superior cytocompatibility in vitro compared to the control hydrogel biomaterials without CaSiO3. Thus, this study revealed that the addition of CaSiO3 to WPI-based hydrogel biomaterials renders them more promising for bone tissue engineering applications.

KW - whey protein

KW - calcium silicate

KW - bone scaffolds

KW - SEM

KW - FTIR

KW - swelling

KW - osteoblasts

KW - cytocompatibility

U2 - 10.3390/ma16196484

DO - 10.3390/ma16196484

M3 - Journal article

VL - 16

JO - Materials

JF - Materials

SN - 1996-1944

IS - 19

M1 - 6484

ER -