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Whole genome sequencing and characterization of a virulent Newcastle disease virus isolated from an outbreak in Sweden

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Whole genome sequencing and characterization of a virulent Newcastle disease virus isolated from an outbreak in Sweden. / Munir, Muhammad; Linde, Anna Malin; Zohari, Siamak et al.

In: Virus Genes, Vol. 43, No. 2, 01.10.2011, p. 261-271.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Munir, M, Linde, AM, Zohari, S, Ståhl, K, Baule, C, Engström, B, M Renström, LH & Berg, M 2011, 'Whole genome sequencing and characterization of a virulent Newcastle disease virus isolated from an outbreak in Sweden', Virus Genes, vol. 43, no. 2, pp. 261-271. https://doi.org/10.1007/s11262-011-0636-2

APA

Munir, M., Linde, A. M., Zohari, S., Ståhl, K., Baule, C., Engström, B., M Renström, L. H., & Berg, M. (2011). Whole genome sequencing and characterization of a virulent Newcastle disease virus isolated from an outbreak in Sweden. Virus Genes, 43(2), 261-271. https://doi.org/10.1007/s11262-011-0636-2

Vancouver

Munir M, Linde AM, Zohari S, Ståhl K, Baule C, Engström B et al. Whole genome sequencing and characterization of a virulent Newcastle disease virus isolated from an outbreak in Sweden. Virus Genes. 2011 Oct 1;43(2):261-271. doi: 10.1007/s11262-011-0636-2

Author

Munir, Muhammad ; Linde, Anna Malin ; Zohari, Siamak et al. / Whole genome sequencing and characterization of a virulent Newcastle disease virus isolated from an outbreak in Sweden. In: Virus Genes. 2011 ; Vol. 43, No. 2. pp. 261-271.

Bibtex

@article{6100fca908c04354b11f9cd0167510e8,
title = "Whole genome sequencing and characterization of a virulent Newcastle disease virus isolated from an outbreak in Sweden",
abstract = "In this study, the complete genome sequence of a Newcastle disease virus (NDV) isolate collected from an outbreak in 1995 in chickens was fully characterized and compared with other NDV sequences. The genome was found to be 15,192 nucleotides long and to consist of six genes in the order 3′-NP-P-M-F-HN-L-5′, similar to other avian paramyxoviruses type-I. However, a six-nucleotide insertion was observed in the 5′ non-coding regions of the nucleoprotein (NP) gene, a feature that is unique to some NDV isolates. The isolate shows the amino acid sequence 112RRQKRF 117 at the cleavage site of the F protein, which is identical to a known motif for virulent pathotypes of NDV. The phylogenetic analysis of the coding region of the F gene indicated that this isolate belongs to genotype VI, more specifically to genotype VId, along with isolates from the other European countries (Denmark, Switzerland and Austria). The same genotype caused outbreaks in the Middle East and Greece in the late 1960s, and in Hungary, in the early 1980s, suggesting a common source for these outbreaks.",
keywords = "Chicken, Complete genome, Newcastle disease virus, Virulent NDV",
author = "Muhammad Munir and Linde, {Anna Malin} and Siamak Zohari and Karl St{\aa}hl and Claudia Baule and Bj{\"o}rn Engstr{\"o}m and {M Renstr{\"o}m}, {Lena H.} and Mikael Berg",
year = "2011",
month = oct,
day = "1",
doi = "10.1007/s11262-011-0636-2",
language = "English",
volume = "43",
pages = "261--271",
journal = "Virus Genes",
issn = "0920-8569",
publisher = "Springer Netherlands",
number = "2",

}

RIS

TY - JOUR

T1 - Whole genome sequencing and characterization of a virulent Newcastle disease virus isolated from an outbreak in Sweden

AU - Munir, Muhammad

AU - Linde, Anna Malin

AU - Zohari, Siamak

AU - Ståhl, Karl

AU - Baule, Claudia

AU - Engström, Björn

AU - M Renström, Lena H.

AU - Berg, Mikael

PY - 2011/10/1

Y1 - 2011/10/1

N2 - In this study, the complete genome sequence of a Newcastle disease virus (NDV) isolate collected from an outbreak in 1995 in chickens was fully characterized and compared with other NDV sequences. The genome was found to be 15,192 nucleotides long and to consist of six genes in the order 3′-NP-P-M-F-HN-L-5′, similar to other avian paramyxoviruses type-I. However, a six-nucleotide insertion was observed in the 5′ non-coding regions of the nucleoprotein (NP) gene, a feature that is unique to some NDV isolates. The isolate shows the amino acid sequence 112RRQKRF 117 at the cleavage site of the F protein, which is identical to a known motif for virulent pathotypes of NDV. The phylogenetic analysis of the coding region of the F gene indicated that this isolate belongs to genotype VI, more specifically to genotype VId, along with isolates from the other European countries (Denmark, Switzerland and Austria). The same genotype caused outbreaks in the Middle East and Greece in the late 1960s, and in Hungary, in the early 1980s, suggesting a common source for these outbreaks.

AB - In this study, the complete genome sequence of a Newcastle disease virus (NDV) isolate collected from an outbreak in 1995 in chickens was fully characterized and compared with other NDV sequences. The genome was found to be 15,192 nucleotides long and to consist of six genes in the order 3′-NP-P-M-F-HN-L-5′, similar to other avian paramyxoviruses type-I. However, a six-nucleotide insertion was observed in the 5′ non-coding regions of the nucleoprotein (NP) gene, a feature that is unique to some NDV isolates. The isolate shows the amino acid sequence 112RRQKRF 117 at the cleavage site of the F protein, which is identical to a known motif for virulent pathotypes of NDV. The phylogenetic analysis of the coding region of the F gene indicated that this isolate belongs to genotype VI, more specifically to genotype VId, along with isolates from the other European countries (Denmark, Switzerland and Austria). The same genotype caused outbreaks in the Middle East and Greece in the late 1960s, and in Hungary, in the early 1980s, suggesting a common source for these outbreaks.

KW - Chicken

KW - Complete genome

KW - Newcastle disease virus

KW - Virulent NDV

U2 - 10.1007/s11262-011-0636-2

DO - 10.1007/s11262-011-0636-2

M3 - Journal article

C2 - 21667282

AN - SCOPUS:80052324920

VL - 43

SP - 261

EP - 271

JO - Virus Genes

JF - Virus Genes

SN - 0920-8569

IS - 2

ER -