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Investigating the lymphatic drainage of the brain: Essential skills and tools

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSNChapter

Published
  • Nazira J. Albargothy
  • Matthew Macgregor Sharp
  • Maureen Gatherer
  • Alan Morris
  • Roy O. Weller
  • Cheryl Hawkes
  • Roxana O. Carare
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Abstract

In this chapter we describe in detail the surgical and imaging techniques employed for the study of the anatomical routes of drainage of cerebrospinal fluid (CSF) and interstitial fluid (ISF) from the brain. The types of tracers, sites of injection, and volumes injected are crucial. For example, when testing the drainage of ISF from the parenchyma, volumes larger than 0.5 μL result in spillage of ISF into the ventricular CSF.