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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Using in-solution digestion, peptide fractionation, and a Q exactive mass spectrometer to analyze the proteome of clathrin-coated vesicles
AU - Borner, Georg H H
AU - Fielding, Andrew B.
N1 - © 2014 Cold Spring Harbor Laboratory Press.
PY - 2014/11/3
Y1 - 2014/11/3
N2 - The characterization of clathrin-coated vesicles (CCVs), including the effects of genetic or biochemical manipulations on their composition, can be studied by mass spectrometry analysis of HeLa cell fractions enriched for CCVs. This protocol describes the preparation of samples by in-solution proteolytic digest and subsequent peptide fractionation, followed by analysis in a Q Exactive mass spectrometer.
AB - The characterization of clathrin-coated vesicles (CCVs), including the effects of genetic or biochemical manipulations on their composition, can be studied by mass spectrometry analysis of HeLa cell fractions enriched for CCVs. This protocol describes the preparation of samples by in-solution proteolytic digest and subsequent peptide fractionation, followed by analysis in a Q Exactive mass spectrometer.
KW - Cell Fractionation
KW - Clathrin-Coated Vesicles
KW - HeLa Cells
KW - Humans
KW - Mass Spectrometry
KW - Proteolysis
KW - Proteome
KW - Journal Article
U2 - 10.1101/pdb.prot084137
DO - 10.1101/pdb.prot084137
M3 - Journal article
C2 - 25368314
VL - 2014
SP - 1192
EP - 1195
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
SN - 1940-3402
IS - 11
ER -