Using in-solution digestion, peptide fractionation, and a Q exactive mass spectrometer to analyze the proteome of clathrin-coated vesicles

Biomedical and Life Sciences

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https://doi.org/10.1101/pdb.prot084137
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Keywords

Cell Fractionation, Clathrin-Coated Vesicles, HeLa Cells, Humans, Mass Spectrometry, Proteolysis, Proteome, Journal Article

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Using in-solution digestion, peptide fractionation, and a Q exactive mass spectrometer to analyze the proteome of clathrin-coated vesicles. / Borner, Georg H H; Fielding, Andrew B.
In: Cold Spring Harbor Protocols, Vol. 2014, No. 11, 03.11.2014, p. 1192-1195.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{a393cb3ed591477298aa26981ac357f0,

title = "Using in-solution digestion, peptide fractionation, and a Q exactive mass spectrometer to analyze the proteome of clathrin-coated vesicles",

abstract = "The characterization of clathrin-coated vesicles (CCVs), including the effects of genetic or biochemical manipulations on their composition, can be studied by mass spectrometry analysis of HeLa cell fractions enriched for CCVs. This protocol describes the preparation of samples by in-solution proteolytic digest and subsequent peptide fractionation, followed by analysis in a Q Exactive mass spectrometer.",

keywords = "Cell Fractionation, Clathrin-Coated Vesicles, HeLa Cells, Humans, Mass Spectrometry, Proteolysis, Proteome, Journal Article",

author = "Borner, {Georg H H} and Fielding, {Andrew B.}",

note = "{\textcopyright} 2014 Cold Spring Harbor Laboratory Press.",

year = "2014",

month = nov,

day = "3",

doi = "10.1101/pdb.prot084137",

language = "English",

volume = "2014",

pages = "1192--1195",

journal = "Cold Spring Harbor Protocols",

issn = "1940-3402",

publisher = "Cold Spring Harbor Laboratory Press",

number = "11",

}

RIS

TY - JOUR

T1 - Using in-solution digestion, peptide fractionation, and a Q exactive mass spectrometer to analyze the proteome of clathrin-coated vesicles

AU - Borner, Georg H H

AU - Fielding, Andrew B.

PY - 2014/11/3

Y1 - 2014/11/3

N2 - The characterization of clathrin-coated vesicles (CCVs), including the effects of genetic or biochemical manipulations on their composition, can be studied by mass spectrometry analysis of HeLa cell fractions enriched for CCVs. This protocol describes the preparation of samples by in-solution proteolytic digest and subsequent peptide fractionation, followed by analysis in a Q Exactive mass spectrometer.

AB - The characterization of clathrin-coated vesicles (CCVs), including the effects of genetic or biochemical manipulations on their composition, can be studied by mass spectrometry analysis of HeLa cell fractions enriched for CCVs. This protocol describes the preparation of samples by in-solution proteolytic digest and subsequent peptide fractionation, followed by analysis in a Q Exactive mass spectrometer.

KW - Cell Fractionation

KW - Clathrin-Coated Vesicles

KW - HeLa Cells

KW - Humans

KW - Mass Spectrometry

KW - Proteolysis

KW - Proteome

KW - Journal Article

U2 - 10.1101/pdb.prot084137

DO - 10.1101/pdb.prot084137

M3 - Journal article

C2 - 25368314

VL - 2014

SP - 1192

EP - 1195

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

SN - 1940-3402

IS - 11

ER -

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