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Using in-solution digestion, peptide fractionation, and a Q exactive mass spectrometer to analyze the proteome of clathrin-coated vesicles

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<mark>Journal publication date</mark>3/11/2014
<mark>Journal</mark>Cold Spring Harbor Protocols
Issue number11
Number of pages4
Pages (from-to)1192-1195
Publication StatusPublished
<mark>Original language</mark>English


The characterization of clathrin-coated vesicles (CCVs), including the effects of genetic or biochemical manipulations on their composition, can be studied by mass spectrometry analysis of HeLa cell fractions enriched for CCVs. This protocol describes the preparation of samples by in-solution proteolytic digest and subsequent peptide fractionation, followed by analysis in a Q Exactive mass spectrometer.