Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
| <mark>Journal publication date</mark> | 3/11/2014 |
|---|---|
| <mark>Journal</mark> | Cold Spring Harbor Protocols |
| Issue number | 11 |
| Volume | 2014 |
| Number of pages | 4 |
| Pages (from-to) | 1192-1195 |
| Publication Status | Published |
| <mark>Original language</mark> | English |
The characterization of clathrin-coated vesicles (CCVs), including the effects of genetic or biochemical manipulations on their composition, can be studied by mass spectrometry analysis of HeLa cell fractions enriched for CCVs. This protocol describes the preparation of samples by in-solution proteolytic digest and subsequent peptide fractionation, followed by analysis in a Q Exactive mass spectrometer.