Home > Research > Publications & Outputs > A phagocytic route for uptake of double-strande...

Research

Links

Text available via DOI:

View graph of relations

A phagocytic route for uptake of double-stranded RNA in RNAi

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

A phagocytic route for uptake of double-stranded RNA in RNAi. / Rocha, João J.E.; Korolchuk, Viktor I.; Robinson, Iain M. et al.
In: PLoS One, Vol. 6, No. 4, e19087, 29.04.2011.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Rocha, JJE, Korolchuk, VI, Robinson, IM & O'Kane, CJ 2011, 'A phagocytic route for uptake of double-stranded RNA in RNAi', PLoS One, vol. 6, no. 4, e19087. https://doi.org/10.1371/journal.pone.0019087

APA

Rocha, J. J. E., Korolchuk, V. I., Robinson, I. M., & O'Kane, C. J. (2011). A phagocytic route for uptake of double-stranded RNA in RNAi. PLoS One, 6(4), Article e19087. https://doi.org/10.1371/journal.pone.0019087

Vancouver

Rocha JJE, Korolchuk VI, Robinson IM, O'Kane CJ. A phagocytic route for uptake of double-stranded RNA in RNAi. PLoS One. 2011 Apr 29;6(4):e19087. doi: 10.1371/journal.pone.0019087

Author

Rocha, João J.E. ; Korolchuk, Viktor I. ; Robinson, Iain M. et al. / A phagocytic route for uptake of double-stranded RNA in RNAi. In: PLoS One. 2011 ; Vol. 6, No. 4.

Bibtex

@article{a3c4646f0b934ebc8196c0d1ec7ec680,
title = "A phagocytic route for uptake of double-stranded RNA in RNAi",
abstract = "RNA interference (RNAi) has a range of physiological functions including as a defence mechanism against viruses. To protect uninfected cells in a multicellular organism, not only a cell-autonomous RNAi response is required but also a systemic one. However, the route of RNA spread in systemic RNAi remains unclear. Here we show that phagocytosis can be a route for double-stranded RNA uptake. Double-stranded RNA expressed in Escherichia coli induces robust RNAi in Drosophila S2 cells, with effectiveness comparable to that of naked dsRNA. We could separate this phagocytic uptake route from that for RNAi induced by naked dsRNA. Therefore, phagocytic uptake of dsRNA offers a potential route for systemic spread of RNAi.",
author = "Rocha, {Jo{\~a}o J.E.} and Korolchuk, {Viktor I.} and Robinson, {Iain M.} and O'Kane, {Cahir J.}",
year = "2011",
month = apr,
day = "29",
doi = "10.1371/journal.pone.0019087",
language = "English",
volume = "6",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "4",

}

RIS

TY - JOUR

T1 - A phagocytic route for uptake of double-stranded RNA in RNAi

AU - Rocha, João J.E.

AU - Korolchuk, Viktor I.

AU - Robinson, Iain M.

AU - O'Kane, Cahir J.

PY - 2011/4/29

Y1 - 2011/4/29

N2 - RNA interference (RNAi) has a range of physiological functions including as a defence mechanism against viruses. To protect uninfected cells in a multicellular organism, not only a cell-autonomous RNAi response is required but also a systemic one. However, the route of RNA spread in systemic RNAi remains unclear. Here we show that phagocytosis can be a route for double-stranded RNA uptake. Double-stranded RNA expressed in Escherichia coli induces robust RNAi in Drosophila S2 cells, with effectiveness comparable to that of naked dsRNA. We could separate this phagocytic uptake route from that for RNAi induced by naked dsRNA. Therefore, phagocytic uptake of dsRNA offers a potential route for systemic spread of RNAi.

AB - RNA interference (RNAi) has a range of physiological functions including as a defence mechanism against viruses. To protect uninfected cells in a multicellular organism, not only a cell-autonomous RNAi response is required but also a systemic one. However, the route of RNA spread in systemic RNAi remains unclear. Here we show that phagocytosis can be a route for double-stranded RNA uptake. Double-stranded RNA expressed in Escherichia coli induces robust RNAi in Drosophila S2 cells, with effectiveness comparable to that of naked dsRNA. We could separate this phagocytic uptake route from that for RNAi induced by naked dsRNA. Therefore, phagocytic uptake of dsRNA offers a potential route for systemic spread of RNAi.

U2 - 10.1371/journal.pone.0019087

DO - 10.1371/journal.pone.0019087

M3 - Journal article

C2 - 21559499

AN - SCOPUS:79955722540

VL - 6

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 4

M1 - e19087

ER -