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An integrated framework for quantifying immune-tumour interactions in a 3D co-culture model

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An integrated framework for quantifying immune-tumour interactions in a 3D co-culture model. / Al-Hity, Gheed; Yang, FengWei; Campillo-Funollet, Eduard et al.
In: Communications Biology, Vol. 4, No. 1, 781, 24.06.2021.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Al-Hity, G, Yang, F, Campillo-Funollet, E, Greenstein, AE, Hunt, H, Mampay, M, Intabli, H, Falcinelli, M, Madzvamuse, A, Venkataraman, C & Flint, MS 2021, 'An integrated framework for quantifying immune-tumour interactions in a 3D co-culture model', Communications Biology, vol. 4, no. 1, 781. https://doi.org/10.1038/s42003-021-02296-7

APA

Al-Hity, G., Yang, F., Campillo-Funollet, E., Greenstein, A. E., Hunt, H., Mampay, M., Intabli, H., Falcinelli, M., Madzvamuse, A., Venkataraman, C., & Flint, M. S. (2021). An integrated framework for quantifying immune-tumour interactions in a 3D co-culture model. Communications Biology, 4(1), Article 781. https://doi.org/10.1038/s42003-021-02296-7

Vancouver

Al-Hity G, Yang F, Campillo-Funollet E, Greenstein AE, Hunt H, Mampay M et al. An integrated framework for quantifying immune-tumour interactions in a 3D co-culture model. Communications Biology. 2021 Jun 24;4(1):781. doi: 10.1038/s42003-021-02296-7

Author

Al-Hity, Gheed ; Yang, FengWei ; Campillo-Funollet, Eduard et al. / An integrated framework for quantifying immune-tumour interactions in a 3D co-culture model. In: Communications Biology. 2021 ; Vol. 4, No. 1.

Bibtex

@article{0b4c365e1d394206af365e01e396ead4,
title = "An integrated framework for quantifying immune-tumour interactions in a 3D co-culture model",
abstract = "Investigational in vitro models that reflect the complexity of the interaction between the immune system and tumours are limited and difficult to establish. Herein, we present a platform to study the tumour-immune interaction using a co-culture between cancer spheroids and activated immune cells. An algorithm was developed for analysis of confocal images of the co-culture to evaluate the following quantitatively; immune cell infiltration, spheroid roundness and spheroid growth. As a proof of concept, the effect of the glucocorticoid stress hormone, cortisol was tested on 66CL4 co-culture model. Results were comparable to 66CL4 syngeneic in vivo mouse model undergoing psychological stress. Furthermore, administration of glucocorticoid receptor antagonists demonstrated the use of this model to determine the effect of treatments on the immune-tumour interplay. In conclusion, we provide a method of quantifying the interaction between the immune system and cancer, which can become a screening tool in immunotherapy design.",
author = "Gheed Al-Hity and FengWei Yang and Eduard Campillo-Funollet and Greenstein, {Andrew E.} and Hazel Hunt and Myrthe Mampay and Haya Intabli and Marta Falcinelli and Anotida Madzvamuse and Chandrasekhar Venkataraman and Flint, {Melanie S.}",
year = "2021",
month = jun,
day = "24",
doi = "10.1038/s42003-021-02296-7",
language = "English",
volume = "4",
journal = "Communications Biology",
publisher = "Nature Research",
number = "1",

}

RIS

TY - JOUR

T1 - An integrated framework for quantifying immune-tumour interactions in a 3D co-culture model

AU - Al-Hity, Gheed

AU - Yang, FengWei

AU - Campillo-Funollet, Eduard

AU - Greenstein, Andrew E.

AU - Hunt, Hazel

AU - Mampay, Myrthe

AU - Intabli, Haya

AU - Falcinelli, Marta

AU - Madzvamuse, Anotida

AU - Venkataraman, Chandrasekhar

AU - Flint, Melanie S.

PY - 2021/6/24

Y1 - 2021/6/24

N2 - Investigational in vitro models that reflect the complexity of the interaction between the immune system and tumours are limited and difficult to establish. Herein, we present a platform to study the tumour-immune interaction using a co-culture between cancer spheroids and activated immune cells. An algorithm was developed for analysis of confocal images of the co-culture to evaluate the following quantitatively; immune cell infiltration, spheroid roundness and spheroid growth. As a proof of concept, the effect of the glucocorticoid stress hormone, cortisol was tested on 66CL4 co-culture model. Results were comparable to 66CL4 syngeneic in vivo mouse model undergoing psychological stress. Furthermore, administration of glucocorticoid receptor antagonists demonstrated the use of this model to determine the effect of treatments on the immune-tumour interplay. In conclusion, we provide a method of quantifying the interaction between the immune system and cancer, which can become a screening tool in immunotherapy design.

AB - Investigational in vitro models that reflect the complexity of the interaction between the immune system and tumours are limited and difficult to establish. Herein, we present a platform to study the tumour-immune interaction using a co-culture between cancer spheroids and activated immune cells. An algorithm was developed for analysis of confocal images of the co-culture to evaluate the following quantitatively; immune cell infiltration, spheroid roundness and spheroid growth. As a proof of concept, the effect of the glucocorticoid stress hormone, cortisol was tested on 66CL4 co-culture model. Results were comparable to 66CL4 syngeneic in vivo mouse model undergoing psychological stress. Furthermore, administration of glucocorticoid receptor antagonists demonstrated the use of this model to determine the effect of treatments on the immune-tumour interplay. In conclusion, we provide a method of quantifying the interaction between the immune system and cancer, which can become a screening tool in immunotherapy design.

U2 - 10.1038/s42003-021-02296-7

DO - 10.1038/s42003-021-02296-7

M3 - Journal article

C2 - 34168276

VL - 4

JO - Communications Biology

JF - Communications Biology

IS - 1

M1 - 781

ER -