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Analysis of patchclamp recordings: model-free multiscale methods and software

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<mark>Journal publication date</mark>9/03/2021
<mark>Journal</mark>European Biophysics Journal
Issue number2
Volume50
Number of pages23
Pages (from-to)187-209
Publication StatusPublished
<mark>Original language</mark>English

Abstract

Analysis of patchclamp recordings is often a challenging issue. We give practical guidance how such recordings can be analyzed using the model-free multiscale idealization methodology JSMURF, JULES, and HILDE. We provide an operational manual how to use the accompanying software available as an R-package and as a graphical user interface. This includes selection of the right approach and tuning of parameters. We also discuss advantages and disadvantages of model-free approaches in comparison to hidden Markov model approaches and explain how they complement each other.