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Application of CRISPR/Cas9 in Understanding Avian Viruses and Developing Poultry Vaccines

Research output: Contribution to Journal/Magazine › Review article › peer-review

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Application of CRISPR/Cas9 in Understanding Avian Viruses and Developing Poultry Vaccines. / Vilela, Julianne; Rohaim, Mohammed A; Munir, Muhammad.
In: Frontiers in cellular and infection microbiology, Vol. 10, 581504, 24.11.2020.

Research output: Contribution to Journal/Magazine › Review article › peer-review

Harvard

Vilela, J, Rohaim, MA & Munir, M 2020, 'Application of CRISPR/Cas9 in Understanding Avian Viruses and Developing Poultry Vaccines', Frontiers in cellular and infection microbiology, vol. 10, 581504. https://doi.org/10.3389/fcimb.2020.581504

APA

Vilela, J., Rohaim, M. A., & Munir, M. (2020). Application of CRISPR/Cas9 in Understanding Avian Viruses and Developing Poultry Vaccines. Frontiers in cellular and infection microbiology, 10, Article 581504. https://doi.org/10.3389/fcimb.2020.581504

Vancouver

Vilela J, Rohaim MA, Munir M. Application of CRISPR/Cas9 in Understanding Avian Viruses and Developing Poultry Vaccines. Frontiers in cellular and infection microbiology. 2020 Nov 24;10:581504. doi: 10.3389/fcimb.2020.581504

Author

Vilela, Julianne ; Rohaim, Mohammed A ; Munir, Muhammad. / Application of CRISPR/Cas9 in Understanding Avian Viruses and Developing Poultry Vaccines. In: Frontiers in cellular and infection microbiology. 2020 ; Vol. 10.

Bibtex

@article{2d9aace7ade2402cb75276bd9d21625a,
title = "Application of CRISPR/Cas9 in Understanding Avian Viruses and Developing Poultry Vaccines",
abstract = "Clustered regularly interspaced short palindromic repeats associated protein nuclease 9 (CRISPR-Cas9) technology offers novel approaches to precisely, cost-effectively, and user-friendly edit genomes for a wide array of applications and across multiple disciplines. This methodology can be leveraged to underpin host-virus interactions, elucidate viral gene functions, and to develop recombinant vaccines. The successful utilization of CRISPR/Cas9 in editing viral genomes has paved the way of developing novel and multiplex viral vectored poultry vaccines. Furthermore, CRISPR/Cas9 can be exploited to rectify major limitations of conventional approaches including reversion to virulent form, recombination with field viruses and transgene, and genome instability. This review provides comprehensive analysis of the potential of CRISPR/Cas9 genome editing technique in understanding avian virus-host interactions and developing novel poultry vaccines. Finally, we discuss the simplest and practical aspects of genome editing approaches in generating multivalent recombinant poultry vaccines that conform simultaneous protection against major avian diseases.",
keywords = "CRISPR/Cas9, avian viruses, recombinant vaccines, genome editing, poultry diseases",
author = "Julianne Vilela and Rohaim, {Mohammed A} and Muhammad Munir",
note = "Copyright {\textcopyright} 2020 Vilela, Rohaim and Munir.",
year = "2020",
month = nov,
day = "24",
doi = "10.3389/fcimb.2020.581504",
language = "English",
volume = "10",
journal = "Frontiers in cellular and infection microbiology",
issn = "2235-2988",
publisher = "NLM (Medline)",

}

RIS

TY - JOUR

T1 - Application of CRISPR/Cas9 in Understanding Avian Viruses and Developing Poultry Vaccines

AU - Vilela, Julianne

AU - Rohaim, Mohammed A

AU - Munir, Muhammad

N1 - Copyright © 2020 Vilela, Rohaim and Munir.

PY - 2020/11/24

Y1 - 2020/11/24

N2 - Clustered regularly interspaced short palindromic repeats associated protein nuclease 9 (CRISPR-Cas9) technology offers novel approaches to precisely, cost-effectively, and user-friendly edit genomes for a wide array of applications and across multiple disciplines. This methodology can be leveraged to underpin host-virus interactions, elucidate viral gene functions, and to develop recombinant vaccines. The successful utilization of CRISPR/Cas9 in editing viral genomes has paved the way of developing novel and multiplex viral vectored poultry vaccines. Furthermore, CRISPR/Cas9 can be exploited to rectify major limitations of conventional approaches including reversion to virulent form, recombination with field viruses and transgene, and genome instability. This review provides comprehensive analysis of the potential of CRISPR/Cas9 genome editing technique in understanding avian virus-host interactions and developing novel poultry vaccines. Finally, we discuss the simplest and practical aspects of genome editing approaches in generating multivalent recombinant poultry vaccines that conform simultaneous protection against major avian diseases.

AB - Clustered regularly interspaced short palindromic repeats associated protein nuclease 9 (CRISPR-Cas9) technology offers novel approaches to precisely, cost-effectively, and user-friendly edit genomes for a wide array of applications and across multiple disciplines. This methodology can be leveraged to underpin host-virus interactions, elucidate viral gene functions, and to develop recombinant vaccines. The successful utilization of CRISPR/Cas9 in editing viral genomes has paved the way of developing novel and multiplex viral vectored poultry vaccines. Furthermore, CRISPR/Cas9 can be exploited to rectify major limitations of conventional approaches including reversion to virulent form, recombination with field viruses and transgene, and genome instability. This review provides comprehensive analysis of the potential of CRISPR/Cas9 genome editing technique in understanding avian virus-host interactions and developing novel poultry vaccines. Finally, we discuss the simplest and practical aspects of genome editing approaches in generating multivalent recombinant poultry vaccines that conform simultaneous protection against major avian diseases.

KW - CRISPR/Cas9

KW - avian viruses

KW - recombinant vaccines

KW - genome editing

KW - poultry diseases

U2 - 10.3389/fcimb.2020.581504

DO - 10.3389/fcimb.2020.581504

M3 - Review article

C2 - 33330126

VL - 10

JO - Frontiers in cellular and infection microbiology

JF - Frontiers in cellular and infection microbiology

SN - 2235-2988

M1 - 581504

ER -