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Colocalization of calcium entry and exocytotic release sites in adrenal chromaffin cells

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Colocalization of calcium entry and exocytotic release sites in adrenal chromaffin cells. / Robinson, Iain M.; Finnegan, Jennifer M.; Monck, Jonathan R. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 7, 28.03.1995, p. 2474-2478.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Robinson, IM, Finnegan, JM, Monck, JR, Wightman, RM & Fernandez, JM 1995, 'Colocalization of calcium entry and exocytotic release sites in adrenal chromaffin cells', Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 7, pp. 2474-2478. https://doi.org/10.1073/pnas.92.7.2474

APA

Robinson, I. M., Finnegan, J. M., Monck, J. R., Wightman, R. M., & Fernandez, J. M. (1995). Colocalization of calcium entry and exocytotic release sites in adrenal chromaffin cells. Proceedings of the National Academy of Sciences of the United States of America, 92(7), 2474-2478. https://doi.org/10.1073/pnas.92.7.2474

Vancouver

Robinson IM, Finnegan JM, Monck JR, Wightman RM, Fernandez JM. Colocalization of calcium entry and exocytotic release sites in adrenal chromaffin cells. Proceedings of the National Academy of Sciences of the United States of America. 1995 Mar 28;92(7):2474-2478. doi: 10.1073/pnas.92.7.2474

Author

Robinson, Iain M. ; Finnegan, Jennifer M. ; Monck, Jonathan R. et al. / Colocalization of calcium entry and exocytotic release sites in adrenal chromaffin cells. In: Proceedings of the National Academy of Sciences of the United States of America. 1995 ; Vol. 92, No. 7. pp. 2474-2478.

Bibtex

@article{a840cfbbe20949649b45a06deed43f99,
title = "Colocalization of calcium entry and exocytotic release sites in adrenal chromaffin cells",
abstract = "'Snapshot' images of localized Ca2+ influx into patch-clamped chromaffin cells were captured by using a recently developed pulsed-laser imaging system. Transient opening of voltage-sensitive Ca2+ channels gave rise to localized elevations of Ca2+ that had the appearance of either 'hotspots' or partial rings found immediately beneath the plasma membrane. When the Ca2+ imaging technique was employed in conjunction with flame-etched carbon-fiber electrodes to spatially map the release sites of catecholamines, it was observed that the sites of Ca2+ entry and catecholamine release were colocalized. These results provide functional support for the idea that secretion occurs from 'active zone'-like structures in neuroendocrine cells.",
keywords = "active zones, amperometry, pulsed-laser Ca imaging, secretion",
author = "Robinson, {Iain M.} and Finnegan, {Jennifer M.} and Monck, {Jonathan R.} and Wightman, {R. Mark} and Fernandez, {Julio M.}",
year = "1995",
month = mar,
day = "28",
doi = "10.1073/pnas.92.7.2474",
language = "English",
volume = "92",
pages = "2474--2478",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "7",

}

RIS

TY - JOUR

T1 - Colocalization of calcium entry and exocytotic release sites in adrenal chromaffin cells

AU - Robinson, Iain M.

AU - Finnegan, Jennifer M.

AU - Monck, Jonathan R.

AU - Wightman, R. Mark

AU - Fernandez, Julio M.

PY - 1995/3/28

Y1 - 1995/3/28

N2 - 'Snapshot' images of localized Ca2+ influx into patch-clamped chromaffin cells were captured by using a recently developed pulsed-laser imaging system. Transient opening of voltage-sensitive Ca2+ channels gave rise to localized elevations of Ca2+ that had the appearance of either 'hotspots' or partial rings found immediately beneath the plasma membrane. When the Ca2+ imaging technique was employed in conjunction with flame-etched carbon-fiber electrodes to spatially map the release sites of catecholamines, it was observed that the sites of Ca2+ entry and catecholamine release were colocalized. These results provide functional support for the idea that secretion occurs from 'active zone'-like structures in neuroendocrine cells.

AB - 'Snapshot' images of localized Ca2+ influx into patch-clamped chromaffin cells were captured by using a recently developed pulsed-laser imaging system. Transient opening of voltage-sensitive Ca2+ channels gave rise to localized elevations of Ca2+ that had the appearance of either 'hotspots' or partial rings found immediately beneath the plasma membrane. When the Ca2+ imaging technique was employed in conjunction with flame-etched carbon-fiber electrodes to spatially map the release sites of catecholamines, it was observed that the sites of Ca2+ entry and catecholamine release were colocalized. These results provide functional support for the idea that secretion occurs from 'active zone'-like structures in neuroendocrine cells.

KW - active zones

KW - amperometry

KW - pulsed-laser Ca imaging

KW - secretion

U2 - 10.1073/pnas.92.7.2474

DO - 10.1073/pnas.92.7.2474

M3 - Journal article

C2 - 7708668

AN - SCOPUS:0028968901

VL - 92

SP - 2474

EP - 2478

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 7

ER -