Final published version
Research output: Contribution to Journal/Magazine › Comment/debate › peer-review
<mark>Journal publication date</mark> | 31/07/1995 |
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<mark>Journal</mark> | Applied and Environmental Microbiology |
Issue number | 7 |
Volume | 61 |
Number of pages | 4 |
Pages (from-to) | 2783-2786 |
Publication Status | Published |
<mark>Original language</mark> | English |
A range of fluorescent viability dyes were used in conjunction with flow cytometry to rapidly enumerate viable bacteria from freshwater environments. Optimal labelling was achieved by using carboxyfluorescein diacetate or chemchrome B with a detergent-mediated permeabilization step. The viable bacterial count under optimal conditions was 7% in oligotrophic lake water and 75% in polluted river water.