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Direct measurements of natural planktonic bacterial community viability by flow cytometry

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Direct measurements of natural planktonic bacterial community viability by flow cytometry. / Porter, J.; Diaper, J.; Edwards, C. et al.
In: Applied and Environmental Microbiology, Vol. 61, No. 7, 31.07.1995, p. 2783-2786.

Research output: Contribution to Journal/MagazineComment/debatepeer-review

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Porter J, Diaper J, Edwards C, Pickup R. Direct measurements of natural planktonic bacterial community viability by flow cytometry. Applied and Environmental Microbiology. 1995 Jul 31;61(7):2783-2786. doi: 10.1128/aem.61.7.2783-2786.1995

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Porter, J. ; Diaper, J. ; Edwards, C. et al. / Direct measurements of natural planktonic bacterial community viability by flow cytometry. In: Applied and Environmental Microbiology. 1995 ; Vol. 61, No. 7. pp. 2783-2786.

Bibtex

@article{886f02f842b84f078124b92fbcb9b632,
title = "Direct measurements of natural planktonic bacterial community viability by flow cytometry",
abstract = "A range of fluorescent viability dyes were used in conjunction with flow cytometry to rapidly enumerate viable bacteria from freshwater environments. Optimal labelling was achieved by using carboxyfluorescein diacetate or chemchrome B with a detergent-mediated permeabilization step. The viable bacterial count under optimal conditions was 7% in oligotrophic lake water and 75% in polluted river water.",
author = "J. Porter and J. Diaper and C. Edwards and R. Pickup",
year = "1995",
month = jul,
day = "31",
doi = "10.1128/aem.61.7.2783-2786.1995",
language = "English",
volume = "61",
pages = "2783--2786",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "7",

}

RIS

TY - JOUR

T1 - Direct measurements of natural planktonic bacterial community viability by flow cytometry

AU - Porter, J.

AU - Diaper, J.

AU - Edwards, C.

AU - Pickup, R.

PY - 1995/7/31

Y1 - 1995/7/31

N2 - A range of fluorescent viability dyes were used in conjunction with flow cytometry to rapidly enumerate viable bacteria from freshwater environments. Optimal labelling was achieved by using carboxyfluorescein diacetate or chemchrome B with a detergent-mediated permeabilization step. The viable bacterial count under optimal conditions was 7% in oligotrophic lake water and 75% in polluted river water.

AB - A range of fluorescent viability dyes were used in conjunction with flow cytometry to rapidly enumerate viable bacteria from freshwater environments. Optimal labelling was achieved by using carboxyfluorescein diacetate or chemchrome B with a detergent-mediated permeabilization step. The viable bacterial count under optimal conditions was 7% in oligotrophic lake water and 75% in polluted river water.

U2 - 10.1128/aem.61.7.2783-2786.1995

DO - 10.1128/aem.61.7.2783-2786.1995

M3 - Comment/debate

AN - SCOPUS:0029012362

VL - 61

SP - 2783

EP - 2786

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 7

ER -