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Drug/bioactive eluting chitosan composite foams for osteochondral tissue engineering

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Drug/bioactive eluting chitosan composite foams for osteochondral tissue engineering. / Samie, Muhammad ; Khan, Ather; Rahman, Saeed et al.
In: International Journal of Biological Macromolecules, Vol. 229, 28.02.2023, p. 561-574.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Samie, M, Khan, A, Rahman, S, Iqbal, H, Yameen, M, Chaudry, A, Galeb, H, Halcovitch, N & Hardy, J 2023, 'Drug/bioactive eluting chitosan composite foams for osteochondral tissue engineering', International Journal of Biological Macromolecules, vol. 229, pp. 561-574. https://doi.org/10.1016/j.ijbiomac.2022.12.293

APA

Samie, M., Khan, A., Rahman, S., Iqbal, H., Yameen, M., Chaudry, A., Galeb, H., Halcovitch, N., & Hardy, J. (2023). Drug/bioactive eluting chitosan composite foams for osteochondral tissue engineering. International Journal of Biological Macromolecules, 229, 561-574. https://doi.org/10.1016/j.ijbiomac.2022.12.293

Vancouver

Samie M, Khan A, Rahman S, Iqbal H, Yameen M, Chaudry A et al. Drug/bioactive eluting chitosan composite foams for osteochondral tissue engineering. International Journal of Biological Macromolecules. 2023 Feb 28;229:561-574. Epub 2023 Jan 5. doi: 10.1016/j.ijbiomac.2022.12.293

Author

Samie, Muhammad ; Khan, Ather ; Rahman, Saeed et al. / Drug/bioactive eluting chitosan composite foams for osteochondral tissue engineering. In: International Journal of Biological Macromolecules. 2023 ; Vol. 229. pp. 561-574.

Bibtex

@article{ff2611d37ac14261b43d9f60f660f280,
title = "Drug/bioactive eluting chitosan composite foams for osteochondral tissue engineering",
abstract = "Joint defects associated with a variety of etiologies often extend deep into the subchondral bone leading to functional impairment and joint immobility, and it is a very challenging task to regenerate the bone-cartilage interface offering significant opportunities for biomaterial-based interventions to improve the quality of life of patients. Herein drug-/bioactive-loaded porous tissue scaffolds incorporating nano-hydroxyapatite (nHAp), chitosan (CS) and either hydroxypropyl methylcellulose (HPMC) or Bombyx mori silk fibroin (SF) are fabricated through freeze drying method as subchondral bone substitute. A combination of spectroscopy and microscopy (Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), energy dispersive X-ray (EDX), and X-ray fluorescence (XRF) were used to analyze the structure of the porous biomaterials. The compressive mechanical properties of these scaffolds are biomimetic of cancellous bone tissues and capable of releasing drugs/bioactives (exemplified with triamcinolone acetonide, TA, or transforming growth factor-β1, TGF-β1, respectively) over a period of days. Mouse preosteoblast MC3T3-E1 cells were observed to adhere and proliferate on the tissue scaffolds as confirmed by the cell attachment, live-dead assay and alamarBlue{\texttrademark} assay. Interestingly, RT-qPCR analysis showed that the TA downregulated inflammatory biomarkers and upregulated the bone-specific biomarkers, suggesting such tissue scaffolds have long-term potential for clinical application.",
keywords = "Chitosan, Composites, Osteogenesis",
author = "Muhammad Samie and Ather Khan and Saeed Rahman and Haffsah Iqbal and Muhammad Yameen and Aqif Chaudry and Hanaa Galeb and Nathan Halcovitch and John Hardy",
year = "2023",
month = feb,
day = "28",
doi = "10.1016/j.ijbiomac.2022.12.293",
language = "English",
volume = "229",
pages = "561--574",
journal = "International Journal of Biological Macromolecules",
issn = "0141-8130",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Drug/bioactive eluting chitosan composite foams for osteochondral tissue engineering

AU - Samie, Muhammad

AU - Khan, Ather

AU - Rahman, Saeed

AU - Iqbal, Haffsah

AU - Yameen, Muhammad

AU - Chaudry, Aqif

AU - Galeb, Hanaa

AU - Halcovitch, Nathan

AU - Hardy, John

PY - 2023/2/28

Y1 - 2023/2/28

N2 - Joint defects associated with a variety of etiologies often extend deep into the subchondral bone leading to functional impairment and joint immobility, and it is a very challenging task to regenerate the bone-cartilage interface offering significant opportunities for biomaterial-based interventions to improve the quality of life of patients. Herein drug-/bioactive-loaded porous tissue scaffolds incorporating nano-hydroxyapatite (nHAp), chitosan (CS) and either hydroxypropyl methylcellulose (HPMC) or Bombyx mori silk fibroin (SF) are fabricated through freeze drying method as subchondral bone substitute. A combination of spectroscopy and microscopy (Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), energy dispersive X-ray (EDX), and X-ray fluorescence (XRF) were used to analyze the structure of the porous biomaterials. The compressive mechanical properties of these scaffolds are biomimetic of cancellous bone tissues and capable of releasing drugs/bioactives (exemplified with triamcinolone acetonide, TA, or transforming growth factor-β1, TGF-β1, respectively) over a period of days. Mouse preosteoblast MC3T3-E1 cells were observed to adhere and proliferate on the tissue scaffolds as confirmed by the cell attachment, live-dead assay and alamarBlue™ assay. Interestingly, RT-qPCR analysis showed that the TA downregulated inflammatory biomarkers and upregulated the bone-specific biomarkers, suggesting such tissue scaffolds have long-term potential for clinical application.

AB - Joint defects associated with a variety of etiologies often extend deep into the subchondral bone leading to functional impairment and joint immobility, and it is a very challenging task to regenerate the bone-cartilage interface offering significant opportunities for biomaterial-based interventions to improve the quality of life of patients. Herein drug-/bioactive-loaded porous tissue scaffolds incorporating nano-hydroxyapatite (nHAp), chitosan (CS) and either hydroxypropyl methylcellulose (HPMC) or Bombyx mori silk fibroin (SF) are fabricated through freeze drying method as subchondral bone substitute. A combination of spectroscopy and microscopy (Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), energy dispersive X-ray (EDX), and X-ray fluorescence (XRF) were used to analyze the structure of the porous biomaterials. The compressive mechanical properties of these scaffolds are biomimetic of cancellous bone tissues and capable of releasing drugs/bioactives (exemplified with triamcinolone acetonide, TA, or transforming growth factor-β1, TGF-β1, respectively) over a period of days. Mouse preosteoblast MC3T3-E1 cells were observed to adhere and proliferate on the tissue scaffolds as confirmed by the cell attachment, live-dead assay and alamarBlue™ assay. Interestingly, RT-qPCR analysis showed that the TA downregulated inflammatory biomarkers and upregulated the bone-specific biomarkers, suggesting such tissue scaffolds have long-term potential for clinical application.

KW - Chitosan

KW - Composites

KW - Osteogenesis

U2 - 10.1016/j.ijbiomac.2022.12.293

DO - 10.1016/j.ijbiomac.2022.12.293

M3 - Journal article

VL - 229

SP - 561

EP - 574

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

ER -