Home > Research > Publications & Outputs > Genomic and biological characterization of Newc...

Electronic data

  • Manuscript for LU

    Rights statement: The final publication is available at Springer via http://dx.doi.org/10.1007/s00705-018-3840-8

    Accepted author manuscript, 1.15 MB, PDF document

    Available under license: CC BY-NC: Creative Commons Attribution-NonCommercial 4.0 International License


Text available via DOI:

View graph of relations

Genomic and biological characterization of Newcastle disease viruses isolated from migratory mallards (Anas platyrhynchos)

Research output: Contribution to journalJournal articlepeer-review

  • Momena Habib
  • Tahir Yaqub
  • Jawad Nazir
  • Wasim Shehzad
  • Aziz-Ul-Rahman
  • Tayyebah Sohail
  • Nadia Mukhtar
  • Arsalan Mehboob
  • Muhammad Munir
  • Muhammad Zubair Shabbir
<mark>Journal publication date</mark>08/2018
<mark>Journal</mark>Archives of Virology
Issue number8
Number of pages10
Pages (from-to)2179-2188
Publication StatusPublished
Early online date30/04/18
<mark>Original language</mark>English


Given the global evolutionary dynamics of Newcastle disease viruses (NDVs), it is imperative to continue extensive surveillance, routine monitoring and characterization of isolates originating from natural reservoirs (waterfowls). In this report, we isolated and characterized two virulent NDV strains from clinically healthy mallard (Anas platyrhynchos). Both isolates had a genome of 15,192 nucleotides encoding six genes in an order of 3´-NP-P-M-F-HN-L-5´. The biological characteristics (mean death time: 49.5-50 hr, EID50108.5 ml-1) and presence of a typical cleavage site in the fusion (F) protein (112R-R-Q-K-R↓F117) confirmed the velogenic nature of these isolates. Phylogenetic analysis classified both isolates as members of genotype VII within class-II. Furthermore, based upon the hypervariable region of the F gene (375 nt), isolates showed clustering within sub-genotype VIIi. Similarity index and parallel comparison revealed a higher nucleotide divergence from commonly used vaccine strains; LaSota (21%) and Mukteswar (17%). A comparative residues analysis with representative strains of different genotypes, including vaccine strains, revealed a number of substitutions at important structural and functional domains within the F and hemagglutinin-neuraminidase (HN) proteins. Together, the results highlight consistent evolution among circulating NDVs supporting extensive surveillance of the virus in waterfowl to better elucidate epidemiology, evolutionary relationships and their impacts on commercial and backyard poultry.

Bibliographic note

The final publication is available at Springer via http://dx.doi.org/10.1007/s00705-018-3840-8