Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
| <mark>Journal publication date</mark> | 3/11/2014 |
|---|---|
| <mark>Journal</mark> | Cold Spring Harbor Protocols |
| Issue number | 11 |
| Volume | 2014 |
| Number of pages | 4 |
| Pages (from-to) | 1184-1187 |
| Publication Status | Published |
| <mark>Original language</mark> | English |
HeLa cell lines can be experimentally manipulated using drugs or gene-silencing techniques such as RNA interference. Fractions enriched for clathrin-coated vesicles (CCVs) can be isolated from these cell lines and used to study the effects of these manipulations on the composition of CCVs. This protocol, originally developed in the laboratory of Margaret Robinson (Cambridge, United Kingdom), describes the preparation of a HeLa cell fraction that is enriched for a mixed population of CCVs and is suitable for analysis by mass spectroscopy, western blotting, or electron microscopy.