Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Isolating HeLa cell fractions enriched for clathrin-coated vesicles
AU - Borner, Georg H H
AU - Fielding, Andrew B.
N1 - © 2014 Cold Spring Harbor Laboratory Press.
PY - 2014/11/3
Y1 - 2014/11/3
N2 - HeLa cell lines can be experimentally manipulated using drugs or gene-silencing techniques such as RNA interference. Fractions enriched for clathrin-coated vesicles (CCVs) can be isolated from these cell lines and used to study the effects of these manipulations on the composition of CCVs. This protocol, originally developed in the laboratory of Margaret Robinson (Cambridge, United Kingdom), describes the preparation of a HeLa cell fraction that is enriched for a mixed population of CCVs and is suitable for analysis by mass spectroscopy, western blotting, or electron microscopy.
AB - HeLa cell lines can be experimentally manipulated using drugs or gene-silencing techniques such as RNA interference. Fractions enriched for clathrin-coated vesicles (CCVs) can be isolated from these cell lines and used to study the effects of these manipulations on the composition of CCVs. This protocol, originally developed in the laboratory of Margaret Robinson (Cambridge, United Kingdom), describes the preparation of a HeLa cell fraction that is enriched for a mixed population of CCVs and is suitable for analysis by mass spectroscopy, western blotting, or electron microscopy.
KW - Blotting, Western
KW - Cell Fractionation
KW - Clathrin-Coated Vesicles
KW - HeLa Cells
KW - Humans
KW - Mass Spectrometry
KW - Microscopy, Electron
KW - Journal Article
U2 - 10.1101/pdb.prot083147
DO - 10.1101/pdb.prot083147
M3 - Journal article
C2 - 25368312
VL - 2014
SP - 1184
EP - 1187
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
SN - 1940-3402
IS - 11
ER -