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  • V protein Production article

    Rights statement: This is the author’s version of a work that was accepted for publication in Journal of Virological Methods. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Virological Methods, 260, 2018 DOI: 10.1016/j.jviromet.2018.07.009

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    Available under license: CC BY-NC-ND: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License

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Production, characterization, and epitope mapping of a monoclonal antibody against genotype VII Newcastle disease virus V protein

Research output: Contribution to journalJournal articlepeer-review

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  • Jihong Li
  • Chunchun Meng
  • Tingting Ren
  • Wei Wang
  • Yaodan Zhang
  • Weifeng Yuan
  • Shuqin Xu
  • Yingjie Sun
  • Lei Tan
  • Cuiping Song
  • Ying Liao
  • Venugopal Nair
  • Muhammad Munir
  • Zhuang Ding
  • Xiufan Liu
  • Xusheng Qiu
  • Chan Ding
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<mark>Journal publication date</mark>1/10/2018
<mark>Journal</mark>Journal of Virological Methods
Volume260
Number of pages10
Pages (from-to)88-97
Publication StatusPublished
Early online date17/07/18
<mark>Original language</mark>English

Abstract

Newcastle disease virus (NDV) V protein is crucial for viral interferon (IFN) antagonism and virulence, determining its host range restriction. However, little information is available on the B cell epitopes of V protein and the subcellular movement of V protein in the process of NDV infection. In this study, the monoclonal antibody (mAb) clone 3D7 against genotype VII NDV V protein was generated by immunizing mice with a purified recombinant His-tagged carboxyl-terminal domain (CTD) region of V protein. Fine epitope mapping analysis and B-cell epitope prediction indicated that mAb 3D7 recognized a linear epitope 152RGPAELWK159, which is located in the V protein CTD region. Sequence alignment showed that the mAb clone 3D7-recognized epitope is highly conserved among Class II genotype VII NDV strains, but not among other genotypes, suggesting it could serve as a genetic marker to differentiate NDV genotypes. Furthermore, the movement of V protein during NDV replication in infected cells were determined by using this mAb. It was found that V protein localized around the nucleus during virus replication. The establishment of V protein-specific mAb and identification of its epitope extend our understanding of the antigenic characteristics of V protein and provide a basis for the development of epitope-based diagnostic assays.

Bibliographic note

This is the author’s version of a work that was accepted for publication in Journal of Virological Methods. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Virological Methods, 260, 2018 DOI: 10.1016/j.jviromet.2018.07.009