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Sequence analysis of Camelpox virus isolated in Egypt

Research output: Contribution to Journal/MagazineJournal articlepeer-review

  • Ayatollah I. Bassioun
  • Soad M. Soliman
  • H. A. Hussein
  • M. A. Rohiam
  • Ahmed A. El-Sanousi
<mark>Journal publication date</mark>2014
<mark>Journal</mark>International Journal of Virology
Issue number2
Number of pages8
Pages (from-to)121-128
Publication StatusPublished
<mark>Original language</mark>English


In this study the collected skin sample from camel suffered from Camelpox virus (CPV) from different regions in Egypt (South Sina Governorate and Maruite camel Farm of the Dessert Research Center) and the propagated isolated virus on Vero cell line were characterized by employing Polymerase Chain Reaction (PCR) and sequencing. The causative agent was identified as CPV, based on A-type inclusion and C18L genes-specific PCRs and partial sequencing of the C18L gene, which clearly confirmed that the outbreaks were caused by CPV. Further, phylogenic analysis of partial C18L gene of the isolated CPV and the Vaccinal strain (Jouf-78) of CPV sequence have showed that the isolated CPV clustered together with other reported isolates of CPV on contrast the vaccinal strain clustered with other vaccinai virus.