Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Sequence analysis of Camelpox virus isolated in Egypt
AU - Bassioun, Ayatollah I.
AU - Soliman, Soad M.
AU - Hussein, H. A.
AU - Rohiam, M. A.
AU - El-Sanousi, Ahmed A.
PY - 2014
Y1 - 2014
N2 - In this study the collected skin sample from camel suffered from Camelpox virus (CPV) from different regions in Egypt (South Sina Governorate and Maruite camel Farm of the Dessert Research Center) and the propagated isolated virus on Vero cell line were characterized by employing Polymerase Chain Reaction (PCR) and sequencing. The causative agent was identified as CPV, based on A-type inclusion and C18L genes-specific PCRs and partial sequencing of the C18L gene, which clearly confirmed that the outbreaks were caused by CPV. Further, phylogenic analysis of partial C18L gene of the isolated CPV and the Vaccinal strain (Jouf-78) of CPV sequence have showed that the isolated CPV clustered together with other reported isolates of CPV on contrast the vaccinal strain clustered with other vaccinai virus.
AB - In this study the collected skin sample from camel suffered from Camelpox virus (CPV) from different regions in Egypt (South Sina Governorate and Maruite camel Farm of the Dessert Research Center) and the propagated isolated virus on Vero cell line were characterized by employing Polymerase Chain Reaction (PCR) and sequencing. The causative agent was identified as CPV, based on A-type inclusion and C18L genes-specific PCRs and partial sequencing of the C18L gene, which clearly confirmed that the outbreaks were caused by CPV. Further, phylogenic analysis of partial C18L gene of the isolated CPV and the Vaccinal strain (Jouf-78) of CPV sequence have showed that the isolated CPV clustered together with other reported isolates of CPV on contrast the vaccinal strain clustered with other vaccinai virus.
KW - C18L gene
KW - Camelpox virus
KW - CPV genome
KW - Farm
KW - PCR
KW - Skin losions
KW - Uaccinei
KW - Verocell line
U2 - 10.3923/ijv.2014.121.128
DO - 10.3923/ijv.2014.121.128
M3 - Journal article
AN - SCOPUS:84898416506
VL - 10
SP - 121
EP - 128
JO - International Journal of Virology
JF - International Journal of Virology
SN - 1816-4900
IS - 2
ER -