Final published version
Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter (peer-reviewed) › peer-review
Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter (peer-reviewed) › peer-review
}
TY - CHAP
T1 - Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts
AU - Sales, CRG
AU - Degen, Gustaf E.
AU - Bernardes Da Silva, Anabela
AU - Carmo-Silva, Ana Elizabete
PY - 2018/7/6
Y1 - 2018/7/6
N2 - Rubisco plays a central role in photosynthesis and, due to its catalytic inefficiencies,frequently limits CO2 assimilation in fully illuminated leaves at the top of unstressed crop canopies.The CO2-fixing enzyme is heavily regulated and not all the enzyme present in the leaf is active at any given moment. In this chapter,a spectrophotometric assay is described for measuring Rubisco activity and activation state in leaf extracts.Most of the assay components are available commercially and others can be produced by established protocols, making adoption of the assay achievable by most plant biochemistry laboratories.Its relative high-throughput capacity enables large-scale experiments aimed at screening germplasm for improved Rubisco function.
AB - Rubisco plays a central role in photosynthesis and, due to its catalytic inefficiencies,frequently limits CO2 assimilation in fully illuminated leaves at the top of unstressed crop canopies.The CO2-fixing enzyme is heavily regulated and not all the enzyme present in the leaf is active at any given moment. In this chapter,a spectrophotometric assay is described for measuring Rubisco activity and activation state in leaf extracts.Most of the assay components are available commercially and others can be produced by established protocols, making adoption of the assay achievable by most plant biochemistry laboratories.Its relative high-throughput capacity enables large-scale experiments aimed at screening germplasm for improved Rubisco function.
M3 - Chapter (peer-reviewed)
SN - 9781493977857
VL - 1770
SP - 239
EP - 250
BT - Photosynthesis
A2 - Covshoff, Sarah
PB - Springer Science + Business Media
CY - Niceville, FL, USA
ER -