TY - JOUR

T1 - Stimulation of secretion from bovine adrenal chromaffin cells by microsecond bursts of therapeutic levels of ultrasound

AU - Robinson, I. M.

AU - Kinnick, R. R.

AU - Greenleaf, J. F.

AU - Fernandez, J. M.

PY - 1996/4/1

Y1 - 1996/4/1

N2 - 1. In this study the secretory response of individual bovine adrenal chromaffin cells was monitored using amperometric carbon-fibre microelectrodes. Cells were stimulated to secrete by exposure to 20-100 μs long tonehursts of ultrasonnd (2-4 x 105 Pa; peak pressure at 1 MHz). 2. Three types of secretory responses were observed: an almost instantaneous response, a delayed release of catecholamines, or a series of 'burst-like' secretory bouts. 3. Fura-2 measurements of intracellular Ca2+ concentrations showed that the release of catecholamines was accompanied by an increase in the intracellular Ca2+ concentration. In the absence of extracellular Ca2+, secretory responses were not evoked showing that Ca2+ entry was necessary to elicit catecholamine release.

AB - 1. In this study the secretory response of individual bovine adrenal chromaffin cells was monitored using amperometric carbon-fibre microelectrodes. Cells were stimulated to secrete by exposure to 20-100 μs long tonehursts of ultrasonnd (2-4 x 105 Pa; peak pressure at 1 MHz). 2. Three types of secretory responses were observed: an almost instantaneous response, a delayed release of catecholamines, or a series of 'burst-like' secretory bouts. 3. Fura-2 measurements of intracellular Ca2+ concentrations showed that the release of catecholamines was accompanied by an increase in the intracellular Ca2+ concentration. In the absence of extracellular Ca2+, secretory responses were not evoked showing that Ca2+ entry was necessary to elicit catecholamine release.

U2 - 10.1113/jphysiol.1996.sp021306

DO - 10.1113/jphysiol.1996.sp021306

M3 - Journal article

C2 - 8730600

AN - SCOPUS:0030006116

VL - 492

SP - 257

EP - 263

JO - Journal of Physiology

JF - Journal of Physiology

SN - 0022-3751

IS - 1

ER -