Home > Research > Publications & Outputs > Structural and functional insights into non-str...

Electronic data

  • Manuscript v2_Revised

    Rights statement: This is the author’s version of a work that was accepted for publication in Microbial Pathogenesis. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Microbial Pathogenesis, 150, 2021 DOI: 10.1016/j.micpath.2020.104641

    Accepted author manuscript, 5.64 MB, PDF document

    Embargo ends: 23/11/21

    Available under license: CC BY-NC-ND

Links

Text available via DOI:

View graph of relations

Structural and functional insights into non-structural proteins of coronaviruses

Research output: Contribution to journalJournal articlepeer-review

Published
Article number104641
<mark>Journal publication date</mark>1/01/2021
<mark>Journal</mark>Microbial pathogenesis
Volume150
Number of pages12
Publication StatusPublished
Early online date23/11/20
<mark>Original language</mark>English

Abstract

Coronaviruses (CoVs) are causing a number of human and animal diseases because of their zoonotic nature such as Middle East respiratory syndrome (MERS), severe acute respiratory syndrome (SARS) and coronavirus disease 2019 (COVID-19). These viruses can infect respiratory, gastrointestinal, hepatic and central nervous systems of human, livestock, birds, bat, mouse, and many wild animals. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a newly emerging respiratory virus and is causing CoVID-19 with high morbidity and considerable mortality. All CoVs belong to the order Nidovirales, family Coronaviridae, are enveloped positive-sense RNA viruses, characterised by club-like spikes on their surfaces and large RNA genome with a distinctive replication strategy. Coronavirus have the largest RNA genomes (~26–32 kilobases) and their expansion was likely enabled by acquiring enzyme functions that counter the commonly high error frequency of viral RNA polymerases. Non-structural proteins (nsp) 7–16 are cleaved from two large replicase polyproteins and guide the replication and processing of coronavirus RNA. Coronavirus replicase has more or less universal activities, such as RNA polymerase (nsp 12) and helicase (nsp 13), as well as a variety of unusual or even special mRNA capping (nsp 14, nsp 16) and fidelity regulation (nsp 14) domains. Besides that, several smaller subunits (nsp 7– nsp 10) serve as essential cofactors for these enzymes and contribute to the emerging “nsp interactome.” In spite of the significant progress in studying coronaviruses structural and functional properties, there is an urgent need to understand the coronaviruses evolutionary success that will be helpful to develop enhanced control strategies. Therefore, it is crucial to understand the structure, function, and interactions of coronaviruses RNA synthesizing machinery and their replication strategies. © 2020

Bibliographic note

This is the author’s version of a work that was accepted for publication in Microbial Pathogenesis. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Microbial Pathogenesis, 150, 2021 DOI: 10.1016/j.micpath.2020.104641