The development of micro-voltammetry to detect the release of secretory products from single cells has yielded surprising information, which suggests that the release of secretory products is regulated after the fusion of secretory vesicles with the plasma membrane. This technique has also been used to demonstrate that the release of secretory products can occur during transient fusion events, which leads one to question the current models for membrane recycling. In the past year, strong evidence has emerged in support of a role for rab3 and Gα_i3 proteins in regulating a putative scaffold of proteins that cause bilayer fusion during exocytosis. These findings parallel the biochemical identification of several new cytosolic, secretory vesicle and plasma membrane proteins that may also play a role in regulating fusion.