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  • VP2_Virus_Like_Particles_elicit_protective_immunity_against_duckling_short_beak_and_dwarfism_syndrome_in_duck_and_chicks

    Rights statement: This is the peer reviewed version of the following article: Xiao, S, Wang, S, Jiang, D, et al. VP2 virus‐like particles elicit protective immunity against duckling short beak and dwarfism syndrome in ducks. Transbound Emerg Dis. 2021; 00: 1– 9. https://doi.org/10.1111/tbed.14021 which has been published in final form at https://onlinelibrary.wiley.com/doi/10.1111/tbed.14021 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

    Accepted author manuscript, 297 KB, PDF document

    Embargo ends: 21/02/22

    Available under license: CC BY-NC: Creative Commons Attribution-NonCommercial 4.0 International License

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VP2 virus-like particles elicit protective immunity against duckling short beak and dwarfism syndrome in ducks

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<mark>Journal publication date</mark>21/02/2021
<mark>Journal</mark>Transboundary and Emerging Diseases
Number of pages9
Publication StatusE-pub ahead of print
Early online date21/02/21
<mark>Original language</mark>English

Abstract

Duckling short beak and dwarfism syndrome virus (SBDSV), an emerging goose parvovirus, has caused short beak and dwarfism syndrome (SBDS) in Chinese duck flocks since 2015. Presently, there is no commercial vaccine against SBDS. In the present study, a virus-like particle (VLP)-based candidate vaccine was developed against this disease. A baculovirus expression system was used to express the SBDSV VP2 protein in Sf9 cells. Immunofluorescence assay, sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting were used to confirm protein expression. Furthermore, transmission electron microscopy was used to observe the formation of VLPs. VLPs were formulated into an oil-adjuvanted maternal vaccine to evaluate humoral responses in breeding ducks via latex particle agglutination inhibition assay (LPAI) and microneutralization assay. The offspring were challenged with SBDSV to test the protective efficacy. A single dose of SBDSV was able to induce the high level of LPAI antibodies in ducks, with LPAI and neutralization peak titres of 4.9 ± 1.20 log2 and 7.1 ± 1.20 log2, respectively, at 4 weeks post-vaccination (wpv). The average LPAI titre of yolk antibodies in duck eggs receiving 2 doses (first and boost doses) of the vaccine was 5.3 ± 1.09 log2 at 4 weeks post-boost. The protective efficacy of the maternal vaccine was 87.5%–100%. These results indicate that SBDSV VLPs can be a promising vaccine candidate for controlling SBDS.

Bibliographic note

This is the peer reviewed version of the following article: Xiao, S, Wang, S, Jiang, D, et al. VP2 virus‐like particles elicit protective immunity against duckling short beak and dwarfism syndrome in ducks. Transbound Emerg Dis. 2021; 00: 1– 9. https://doi.org/10.1111/tbed.14021 which has been published in final form at https://onlinelibrary.wiley.com/doi/10.1111/tbed.14021 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.