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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Avian Sarcoma/Leukosis Virus (RCAS)-mediated Over-expression of IFITM3 Protects Chicks from Highly Pathogenic Avian Influenza Virus Subtype H5N1
AU - Rohaim, Mohammed A.
AU - Gardiner, Emma Louise
AU - Naggar, Rania F. El
AU - Abdelsabour, Mohammed A.
AU - Madbouly, Yahia M.
AU - Atasoy, Mustafa O.
AU - Ahmed, Kawkab A.
AU - El-Safty, Munir M.
AU - Munir, Muhammad
PY - 2024/2/28
Y1 - 2024/2/28
N2 - Broad-spectrum antiviral activities of interferon-induced transmembrane proteins (IFITMs) are primarily attributed to in vitro inhibition of viral entry. Here, we used an avian sarcoma-leukosis virus (RCAS)-based gene transfer system and successfully generated chicks that constitutively express chicken IFITM3 (chIFITM3). The chIFITM3-overexpressing chicks showed significant protection and disease tolerance against highly pathogenic avian influenza virus (HPAIV) H5N1 (Clade 2.2.1.2). The chicks, overexpressing chIFITM3, also showed delayed onset of clinical symptoms, reduced viral shedding, and alleviated histopathologic alterations compared to control and challenged chicks. These findings highlight that overexpression of chIFITM3 provide a substantial defense against zoonotic H5N1 in vivo.
AB - Broad-spectrum antiviral activities of interferon-induced transmembrane proteins (IFITMs) are primarily attributed to in vitro inhibition of viral entry. Here, we used an avian sarcoma-leukosis virus (RCAS)-based gene transfer system and successfully generated chicks that constitutively express chicken IFITM3 (chIFITM3). The chIFITM3-overexpressing chicks showed significant protection and disease tolerance against highly pathogenic avian influenza virus (HPAIV) H5N1 (Clade 2.2.1.2). The chicks, overexpressing chIFITM3, also showed delayed onset of clinical symptoms, reduced viral shedding, and alleviated histopathologic alterations compared to control and challenged chicks. These findings highlight that overexpression of chIFITM3 provide a substantial defense against zoonotic H5N1 in vivo.
KW - Chicken
KW - H5N1
KW - IFITM3
KW - ISG
KW - Influenza
U2 - 10.1016/j.micinf.2023.105231
DO - 10.1016/j.micinf.2023.105231
M3 - Journal article
VL - 26
JO - Microbes and Infection
JF - Microbes and Infection
SN - 1286-4579
IS - 1-2
M1 - 105231
ER -