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Avian Sarcoma/Leukosis Virus (RCAS)-mediated Over-expression of IFITM3 Protects Chicks from Highly Pathogenic Avian Influenza Virus Subtype H5N1

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Avian Sarcoma/Leukosis Virus (RCAS)-mediated Over-expression of IFITM3 Protects Chicks from Highly Pathogenic Avian Influenza Virus Subtype H5N1. / Rohaim, Mohammed A.; Gardiner, Emma Louise; Naggar, Rania F. El et al.
In: Microbes and Infection, Vol. 26, No. 1-2, 105231, 28.02.2024.

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Rohaim MA, Gardiner EL, Naggar RFE, Abdelsabour MA, Madbouly YM, Atasoy MO et al. Avian Sarcoma/Leukosis Virus (RCAS)-mediated Over-expression of IFITM3 Protects Chicks from Highly Pathogenic Avian Influenza Virus Subtype H5N1. Microbes and Infection. 2024 Feb 28;26(1-2):105231. Epub 2023 Sept 28. doi: 10.1016/j.micinf.2023.105231

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@article{277bd2450eea4f8aadf6d6fb077115cb,
title = "Avian Sarcoma/Leukosis Virus (RCAS)-mediated Over-expression of IFITM3 Protects Chicks from Highly Pathogenic Avian Influenza Virus Subtype H5N1",
abstract = "Broad-spectrum antiviral activities of interferon-induced transmembrane proteins (IFITMs) are primarily attributed to in vitro inhibition of viral entry. Here, we used an avian sarcoma-leukosis virus (RCAS)-based gene transfer system and successfully generated chicks that constitutively express chicken IFITM3 (chIFITM3). The chIFITM3-overexpressing chicks showed significant protection and disease tolerance against highly pathogenic avian influenza virus (HPAIV) H5N1 (Clade 2.2.1.2). The chicks, overexpressing chIFITM3, also showed delayed onset of clinical symptoms, reduced viral shedding, and alleviated histopathologic alterations compared to control and challenged chicks. These findings highlight that overexpression of chIFITM3 provide a substantial defense against zoonotic H5N1 in vivo.",
keywords = "Chicken, H5N1, IFITM3, ISG, Influenza",
author = "Rohaim, {Mohammed A.} and Gardiner, {Emma Louise} and Naggar, {Rania F. El} and Abdelsabour, {Mohammed A.} and Madbouly, {Yahia M.} and Atasoy, {Mustafa O.} and Ahmed, {Kawkab A.} and El-Safty, {Munir M.} and Muhammad Munir",
year = "2024",
month = feb,
day = "28",
doi = "10.1016/j.micinf.2023.105231",
language = "English",
volume = "26",
journal = "Microbes and Infection",
issn = "1286-4579",
publisher = "Elsevier Masson SAS",
number = "1-2",

}

RIS

TY - JOUR

T1 - Avian Sarcoma/Leukosis Virus (RCAS)-mediated Over-expression of IFITM3 Protects Chicks from Highly Pathogenic Avian Influenza Virus Subtype H5N1

AU - Rohaim, Mohammed A.

AU - Gardiner, Emma Louise

AU - Naggar, Rania F. El

AU - Abdelsabour, Mohammed A.

AU - Madbouly, Yahia M.

AU - Atasoy, Mustafa O.

AU - Ahmed, Kawkab A.

AU - El-Safty, Munir M.

AU - Munir, Muhammad

PY - 2024/2/28

Y1 - 2024/2/28

N2 - Broad-spectrum antiviral activities of interferon-induced transmembrane proteins (IFITMs) are primarily attributed to in vitro inhibition of viral entry. Here, we used an avian sarcoma-leukosis virus (RCAS)-based gene transfer system and successfully generated chicks that constitutively express chicken IFITM3 (chIFITM3). The chIFITM3-overexpressing chicks showed significant protection and disease tolerance against highly pathogenic avian influenza virus (HPAIV) H5N1 (Clade 2.2.1.2). The chicks, overexpressing chIFITM3, also showed delayed onset of clinical symptoms, reduced viral shedding, and alleviated histopathologic alterations compared to control and challenged chicks. These findings highlight that overexpression of chIFITM3 provide a substantial defense against zoonotic H5N1 in vivo.

AB - Broad-spectrum antiviral activities of interferon-induced transmembrane proteins (IFITMs) are primarily attributed to in vitro inhibition of viral entry. Here, we used an avian sarcoma-leukosis virus (RCAS)-based gene transfer system and successfully generated chicks that constitutively express chicken IFITM3 (chIFITM3). The chIFITM3-overexpressing chicks showed significant protection and disease tolerance against highly pathogenic avian influenza virus (HPAIV) H5N1 (Clade 2.2.1.2). The chicks, overexpressing chIFITM3, also showed delayed onset of clinical symptoms, reduced viral shedding, and alleviated histopathologic alterations compared to control and challenged chicks. These findings highlight that overexpression of chIFITM3 provide a substantial defense against zoonotic H5N1 in vivo.

KW - Chicken

KW - H5N1

KW - IFITM3

KW - ISG

KW - Influenza

U2 - 10.1016/j.micinf.2023.105231

DO - 10.1016/j.micinf.2023.105231

M3 - Journal article

VL - 26

JO - Microbes and Infection

JF - Microbes and Infection

SN - 1286-4579

IS - 1-2

M1 - 105231

ER -